Ikeda G J, Miller E, Sapienza P P, Michel T C, Inskeep P B
Division of Toxicology, Food and Drug Administration, Washington, DC 20204.
Food Chem Toxicol. 1987 Nov;25(11):871-5. doi: 10.1016/0278-6915(87)90267-5.
Male beagle dogs and miniature pigs were given acrylamide in the diet for 3-4 wk at a dosage of 1 mg/kg/day. They were then given [1-14C]acrylamide as a single oral dose of 1 mg/kg. The animals were killed 6 hr or 1, 2, 4 or 14 days after administration of the radioactive compound and tissues were analysed for radioactivity. The radiolabelled material was distributed to a major extent in muscle tissue in both species (31-35% of the dose at 6 hr and 5-7% at 14 days). Although the nervous system is the primary target for acrylamide monomer toxicity, less than 1% of the administered 14C was found in the brain in both species. No neurotoxic signs were evident during the exposure period at the dosage used. Analysis of discrete areas of the brain for radioactivity revealed that the levels of penetration of [1-14C]acrylamide in brain paralleled the vascularization pattern of the tissues. Approximately 60% of the administered radiolabel was excreted in the urine in both species and smaller amounts were excreted in the faeces. However, recovery in the faeces was higher in pigs (c. 25%) than in dogs (c. 7%) and this and the considerably higher levels demonstrated in the gastro-intestinal tract of the pigs indicated that the absorption of acrylamide was more rapid and more extensive in dogs than in pigs.
雄性比格犬和小型猪在饮食中以1毫克/千克/天的剂量摄入丙烯酰胺，持续3 - 4周。然后它们接受[1 - 14C]丙烯酰胺，单次口服剂量为1毫克/千克。在给予放射性化合物后的6小时、1天、2天、4天或14天处死动物，并分析组织中的放射性。在这两个物种中，放射性标记物质主要分布在肌肉组织中（6小时时占剂量的31 - 35%，14天时占5 - 7%）。尽管神经系统是丙烯酰胺单体毒性的主要靶器官，但在这两个物种的大脑中发现，摄入的14C不到1%。在所使用的剂量下，暴露期间未出现明显的神经毒性迹象。对大脑离散区域的放射性分析表明，[1 - 14C]丙烯酰胺在大脑中的渗透水平与组织的血管分布模式平行。在这两个物种中，约60%的摄入放射性标记物经尿液排出，少量经粪便排出。然而，猪粪便中的回收率（约25%）高于狗（约7%），这以及猪胃肠道中显示出的显著更高水平表明，丙烯酰胺在狗体内的吸收比在猪体内更快、更广泛。
