Ikeda G J, Miller E, Sapienza P P, Michel T C, King M T, Turner V A, Blumenthal H, Jackson W E, Levin S
Food Chem Toxicol. 1983 Feb;21(1):49-58. doi: 10.1016/0278-6915(83)90268-5.
[14C]Acrylamide and [14C]betaine hydrochloride were administered in a single iv dose to pregnant rats, rabbits, beagle dogs and miniature pigs late in gestation (1-2 days before expected parturition). Dosages used were 10 mg/kg for rats and 5 mg/kg for the other species. The compounds were allowed to equilibrate in the animal (for 1 hr in rats and for 2 hr in the other species); the dam was then killed and the foetuses were removed by caesarean section. Each foetus was weighed and analysed for radioactivity, either by homogenization of the whole foetus (rat and rabbit) or by determining separately the radioactivity in individual organs and tissues (dog and pig). Foetal uptake of the polar compound betaine hydrochloride was much lower than that of the more lipophilic acrylamide. The sex of the foetus did not appear to affect uptake of either compound. There were no significant differences in total uptake of isotope attributable to the position of the foetus within the uterus in any of the four species given either acrylamide or betaine. Similarly, uterine position did not affect the uptake of acrylamide or betaine by individual tissues of foetal dogs or pigs. Since the distributions of 14C-labelled acrylamide and betaine hydrochloride were essentially uniform throughout a litter, it would not be necessary to sample all of the members of a litter to obtain a representative picture of foetal distribution.
在妊娠后期（预计分娩前1 - 2天），给怀孕的大鼠、兔子、比格犬和小型猪静脉注射单次剂量的[¹⁴C]丙烯酰胺和[¹⁴C]盐酸甜菜碱。大鼠的使用剂量为10 mg/kg，其他物种为5 mg/kg。使化合物在动物体内达到平衡（大鼠平衡1小时，其他物种平衡2小时）；然后处死母兽，通过剖腹产取出胎儿。称取每个胎儿的体重，并通过将整个胎儿匀浆（大鼠和兔子）或分别测定各个器官和组织中的放射性（狗和猪）来分析放射性。极性化合物盐酸甜菜碱的胎儿摄取量远低于亲脂性更强的丙烯酰胺。胎儿的性别似乎不影响这两种化合物的摄取。在给予丙烯酰胺或盐酸甜菜碱的四种物种中，任何一种物种的胎儿在子宫内的位置对同位素的总摄取量均无显著差异。同样，子宫位置也不影响胎儿狗或猪的各个组织对丙烯酰胺或盐酸甜菜碱的摄取。由于¹⁴C标记的丙烯酰胺和盐酸甜菜碱在一窝胎儿中的分布基本均匀，因此无需对一窝中的所有个体进行采样就能获得胎儿分布的代表性图像。
