Ikeda G J, Miller E, Sapienza P P, Michel T C, King M T, Sager A O
Food Chem Toxicol. 1985 Aug;23(8):757-61. doi: 10.1016/0278-6915(85)90271-6.
[carbonyl-14C]Acrylamide was administered iv as a single dose (5 mg/kg) to pregnant beagle dogs and miniature pigs late in gestation. After a 2-hr equilibration period, the animals were killed and foetuses were removed for determination of the amount of radioactivity in maternal and foetal tissues. In total, six dog litters (33 foetuses) and seven pig litters (45 foetuses) were examined. In dogs, acrylamide was distributed readily to both maternal and foetal tissues with a placental distribution factor of 17.7%. The blood/brain distribution factor was insignificant (5.9%) in maternal dogs and 0% in the foetuses. Maternal liver was the largest depot of the administered acrylamide in the dog, followed by the maternal kidney. In pigs, the placental distribution factor was 31%, and the blood/brain distribution factor was insignificant in both maternal and foetal pigs. Liver and kidney of maternal pigs also contained the greatest amount of radioactivity. Although there appears to be some placental protection of the foetuses from the xenobiotic in the maternal circulation, foetal brain would be exposed to the effect of any acrylamide present in the foetal circulation, since the foetuses of both species had blood/brain distribution factors that were either small or zero, reflecting the absence of a blood-brain barrier.
在妊娠后期，将[羰基-14C]丙烯酰胺以单剂量（5毫克/千克）静脉注射给怀孕的比格犬和小型猪。经过2小时的平衡期后，处死动物并取出胎儿，以测定母体和胎儿组织中的放射性量。总共检查了6窝犬（33只胎儿）和7窝猪（45只胎儿）。在犬中，丙烯酰胺很容易分布到母体和胎儿组织中，胎盘分布因子为17.7%。母体犬的血脑分布因子不显著（5.9%），胎儿的血脑分布因子为0%。母体肝脏是犬体内注射的丙烯酰胺的最大储存部位，其次是母体肾脏。在猪中，胎盘分布因子为31%，母体和胎儿猪的血脑分布因子均不显著。母体猪的肝脏和肾脏也含有最大量的放射性。虽然似乎有一定的胎盘屏障可保护胎儿免受母体循环中外源生物的影响，但由于两种动物的胎儿血脑分布因子都很小或为零，反映出缺乏血脑屏障，胎儿脑仍会受到胎儿循环中任何丙烯酰胺的影响。
