Lang Shiying, Du Yangrui, Ma Li, Bai Yangjing, Ji Ying, Liu Gongyan
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
West China School of Nursing, Sichuan University/Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Biomacromolecules. 2023 Apr 10;24(4):1839-1854. doi: 10.1021/acs.biomac.3c00043. Epub 2023 Mar 16.
Hemostatic powders provide an important treatment approach for time-sensitive hemorrhage control. Conventional hemostatic powders are challenged by the lack of tissue adhesiveness, insufficient hemostatic efficacy, limited infection control, and so forth. This study develops a hemostatic powder from tricomponent GTP coacervates consisting of gelatin, tannic acid (TA), and poly(vinyl alcohol) (PVA). The physical cross-linking by TA results in facile preparation, good storage stability, ease of application to wounds, and removal, which provide good potential for clinical translation. When rehydrated, the coacervate powders rapidly form a cohesive layer with interconnected microporous structure, competent flexibility, switchable wet adhesiveness, and antibacterial properties, which facilitate the hemostatic efficacy for treating irregular, noncompressible, or bacteria-infected wounds. Compared to commercial hemostats, GTP treatment results in significantly accelerated hemostasis in a liver puncture model (∼19 s, >30% reduction in the hemostatic time) and in a tail amputation model (∼38 s, >60% reduction in the hemostatic time). In the GTP coacervates, gelatin functioned as the biodegradable scaffold, while PVA introduced the flexible segments to enable shape-adaptability and interfacial interactions. Furthermore, TA contributed to the physical cross-linking, adhesiveness, and antibacterial performance of the coacervates. The study explores the tunability of GTP coacervate powders to enhance their hemostatic and wound healing performances.
止血粉为时间敏感型出血控制提供了一种重要的治疗方法。传统止血粉面临着组织粘附性不足、止血效果欠佳、感染控制有限等挑战。本研究利用由明胶、单宁酸（TA）和聚乙烯醇（PVA）组成的三组分GTP凝聚层开发了一种止血粉。TA引起的物理交联使得制备简便、储存稳定性良好、易于应用于伤口以及易于去除，这为临床转化提供了良好的潜力。复水后，凝聚层粉末迅速形成具有相互连接的微孔结构、良好柔韧性、可切换的湿粘附性和抗菌性能的凝聚层，这有助于治疗不规则、不可压缩或细菌感染伤口的止血效果。与市售止血剂相比，在肝穿刺模型（约19秒，止血时间减少>30%）和尾部截肢模型（约38秒，止血时间减少>60%）中，GTP处理可显著加速止血。在GTP凝聚层中，明胶作为可生物降解的支架，而PVA引入了柔性链段以实现形状适应性和界面相互作用。此外，TA有助于凝聚层的物理交联、粘附性和抗菌性能。该研究探索了GTP凝聚层粉末的可调节性，以增强其止血和伤口愈合性能。
