Du Yangrui, Bai Yangjing, Lang Shiying, Xing Dandan, Ma Li, Li Kaijun, Peng Jinyu, Li Xinyun, Liu Gongyan
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
West China School of Nursing, Sichuan University/Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Biomacromolecules. 2023 Nov 13;24(11):5313-5327. doi: 10.1021/acs.biomac.3c00803. Epub 2023 Sep 19.
Developing a hemostatic sponge that can effectively control bleeding from visceral injuries while guiding in situ tissue regeneration in incompressible wounds remains a challenge. Most of the existing hemostatic sponges degrade slowly, are relatively single-functioning, and cannot cope with complex environments. Herein, a biodegradable rapidly hemostatic sponge (GPZ) was created by dual-dynamic-bond cross-linking among Zn, protocatechualdehyde (PA)-containing catechol and aldehyde groups, and gelatin. GPZ had a uniformly distributed interconnected pore structure with excellent fluid absorption. It could effectively absorb the oozing blood and increase the blood concentration while stimulating platelet activation and accelerating blood coagulation. Compared to commercial hemostats, GPZ treatment significantly accelerated hemostasis in the rat liver defect model (∼0.33 min, ≥50% reduction in the hemostatic time) and in the rabbit liver defect model (∼1.02 min, ≥60% reduction in the hemostatic time). Additionally, GPZ had excellent antibacterial and antioxidant properties that effectively protected the wound from infection and excessive inflammation. In the liver regeneration model, GPZ significantly increased the rate of hepatic tissue repair and promoted rapid functional recovery without complications and adverse reactions. Overall, we designed a simple and effective biodegradable rapid hemostatic sponge with good clinical translational potential for treating lethal incompressible bleeding and promoting wound healing.
开发一种能够有效控制内脏损伤出血，同时引导不可压缩伤口原位组织再生的止血海绵仍然是一项挑战。现有的大多数止血海绵降解缓慢，功能相对单一，无法应对复杂环境。在此，通过锌、含原儿茶醛（PA）的儿茶酚和醛基以及明胶之间的双动态键交联，制备了一种可生物降解的快速止血海绵（GPZ）。GPZ具有均匀分布的相互连通的孔隙结构，吸液性能优异。它能有效吸收渗出的血液，提高血液浓度，同时刺激血小板活化，加速血液凝固。与市售止血剂相比，在大鼠肝缺损模型（约0.33分钟，止血时间减少≥50%）和兔肝缺损模型（约1.02分钟，止血时间减少≥60%）中，GPZ治疗显著加速了止血。此外，GPZ具有优异的抗菌和抗氧化性能，能有效保护伤口免受感染和过度炎症。在肝再生模型中，GPZ显著提高了肝组织修复率，促进了快速功能恢复，且无并发症和不良反应。总体而言，我们设计了一种简单有效的可生物降解快速止血海绵，具有良好的临床转化潜力，可用于治疗致命性不可压缩出血并促进伤口愈合。
