通过黄腐酸控制冰晶形成的红细胞冷冻保存方案。

Protocol for cryopreservation of red blood cells that controls ice crystal formation by fulvic acid.

作者信息

Hu Jinhao, Zhao Xinqi, Bai Guoying

机构信息

School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300401, China.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300401, China; Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

出版信息

STAR Protoc. 2023 Mar 14;4(2):102160. doi: 10.1016/j.xpro.2023.102160.

DOI:10.1016/j.xpro.2023.102160

PMID:36924506

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10026024/

Abstract

The development of biocompatible ice-controlling materials for non-vitreous cryopreservation of cells is of great importance to the field of biomedicine. Here, we present a protocol to use fulvic acid (FA) for efficient non-vitreous cryopreservation of red blood cells (RBCs) that both promotes the melting of ice crystals and retards their growth/recrystallization. We describe steps for FA fractionation and performing tests for ice recrystallization and ice freezing/thawing. We then detail the freezing/thawing of RBCs, recovering RBCs, and testing their viability. For complete details on the use and execution of this protocol, please refer to Bai et al. (2022)..

摘要

开发用于细胞非玻璃化冷冻保存的生物相容性控冰材料对生物医学领域至关重要。在此，我们提出一种使用富里酸（FA）对红细胞（RBC）进行高效非玻璃化冷冻保存的方案，该方案既能促进冰晶融化，又能延缓其生长/重结晶。我们描述了FA分级分离步骤以及进行冰重结晶和冰冷冻/解冻测试的步骤。然后，我们详细介绍了RBC的冷冻/解冻、RBC回收以及测试其活力的过程。有关此方案的使用和执行的完整详细信息，请参考Bai等人（2022年）的文献。