Lee Yuri, Choi Sooji, Kim Kyung Won
Department of Life Science, Hallym University, Chuncheon 24252, South Korea.
Department of Life Science, Hallym University, Chuncheon 24252, South Korea; Multidisciplinary Genome Institute, Hallym University, Chuncheon 24252, South Korea.
Ecotoxicol Environ Saf. 2023 Apr 15;255:114752. doi: 10.1016/j.ecoenv.2023.114752. Epub 2023 Mar 14.
Dithianon is a conventional broad-spectrum protectant fungicide widely used in agriculture, but its potential neurotoxic risk to animals remains largely unknown. In this study, neurotoxic effects of Dithianon and its underlying cellular and molecular mechanisms were investigated using the nematode, Caenorhabditis elegans, as a model system. Upon chronic exposure of C. elegans to Dithianon, dopaminergic neurons were found to be vulnerable, with significant degeneration in terms of structure and function in a concentration-dependent manner. In examining toxicity mechanisms, we observed significant Dithianon-induced increases in oxidative stress and mitochondrial fragmentation, both of which are often associated with cellular stress. The present study suggests that Dithianon exposure causes dopaminergic neurotoxicity in C. elegans, by inducing oxidative stress and mitochondrial dysfunction. These findings contribute to a better understanding of Dithianon's neurotoxic potential.
二噻农是一种广泛应用于农业的传统广谱保护性杀菌剂，但其对动物潜在的神经毒性风险在很大程度上仍不为人知。在本研究中，以线虫秀丽隐杆线虫为模型系统，研究了二噻农的神经毒性作用及其潜在的细胞和分子机制。秀丽隐杆线虫长期暴露于二噻农后，发现多巴胺能神经元易受影响，其结构和功能在浓度依赖的方式下发生显著退化。在研究毒性机制时，我们观察到二噻农显著诱导氧化应激增加和线粒体碎片化，这两者通常都与细胞应激有关。本研究表明，暴露于二噻农会通过诱导氧化应激和线粒体功能障碍，导致秀丽隐杆线虫多巴胺能神经毒性。这些发现有助于更好地理解二噻农的神经毒性潜力。
