Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey; University of Health Sciences, Gülhane Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey.
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey; Ankara University, Graduate School of Health Sciences, Ankara, Turkey.
Bioelectrochemistry. 2023 Aug;152:108411. doi: 10.1016/j.bioelechem.2023.108411. Epub 2023 Mar 11.
This study represents nanoparticle-based well-oriented recognition sites via interface imprinting, followed by selective and sensitive determination of fluoxetine (FLX). Herein, FLX was firstly immobilized onto ZnO NPs, and then polymerization was carried out with MAPA, HEMA, and EGDMA on the glassy carbon electrode via photopolymerization. After the etching of ZnO with and 10 mM HCI solution, a porous structure with recognition sites for FLX was constructed onto surface. The characterization of the electrochemical sensor was accomplished by utilizing CV, EIS, ATR-FTIR AFM, and SEM analysis. The DPV was used to determine FLX in standard solution, serum sample, and tap water. The effect of FLX concentration variation was studied using the DPV in the range of 1.0 × 10 M to 1.0 × 10 M with a detection limit of 2.67 × 10 M. This sensor showed specific recognition toward template, and more than 90% of its original response was retained after being stored in the desiccator at R.T. for 5 days. This technique has proven to be a powerful, highly selective, and sensitive tool for the rapid detection of FLX in tap water and spike serum samples.
本研究通过界面印迹，制备了基于纳米粒子的定向识别位点，随后对氟西汀（FLX）进行了选择性和灵敏的测定。在此，首先将 FLX 固定在 ZnO NPs 上，然后通过光聚合，在玻碳电极上用 MAPA、HEMA 和 EGDMA 进行聚合。用 10 mM HCI 溶液刻蚀 ZnO 后，在表面构建了具有 FLX 识别位点的多孔结构。通过 CV、EIS、ATR-FTIR、AFM 和 SEM 分析对电化学传感器进行了表征。使用 DPV 在标准溶液、血清样品和自来水中测定 FLX。使用 DPV 研究了 FLX 浓度变化的影响，其范围为 1.0×10 M 至 1.0×10 M，检测限为 2.67×10 M。该传感器对模板具有特异性识别，在室温下储存在干燥器中 5 天后，其原始响应保留超过 90%。该技术已被证明是一种快速检测自来水中和加标血清样品中 FLX 的强大、高选择性和灵敏的工具。
