Borowska Magdalena, Jiménez-Lamana Javier, Bierla Katarzyna, Jankowski Krzysztof, Szpunar Joanna
Warsaw University of Technology, Faculty of Chemistry, Chair of Analytical Chemistry, 00-664 Warsaw, Poland.
Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM, UMR 5254, Pau, France.
Food Chem. 2023 Aug 15;417:135864. doi: 10.1016/j.foodchem.2023.135864. Epub 2023 Mar 6.
We present a novel microwave-assisted green synthesis of selenium nanoparticles (SeNPs) using yeast extract as source of a non-toxic reducing and capping agents. Effects of synthesis and gastrointestinal digestion conditions on the biogenic Se particle size distribution and number concentration using SP ICP MS were evaluated. The median equivalent diameter of SeNPs varied depending on the synthesis conditions. Upon incubation in simulated gastric juice, the increase of SeNPs size was observed, whereas after simulated intestinal juice addition, their size came back close to the initial value. The biomolecules contained in yeast extract, which play predominant role in the synthesis of SeNPs, were identified by non-targeted qualitative analysis using LC Orbitrap ESI MS. The use of the state-of-the-art MS techniques allowed both the comprehensive assessment of the processes leading to the SeNPs formation and the evaluation of their behavior under gastrointestinal conditions which is of utmost importance for their use as a novel selenium source.
我们提出了一种新型的微波辅助绿色合成硒纳米颗粒（SeNPs）的方法，该方法使用酵母提取物作为无毒还原剂和封端剂的来源。利用单颗粒电感耦合等离子体质谱（SP ICP MS）评估了合成条件和胃肠消化条件对生物源硒颗粒尺寸分布和数量浓度的影响。SeNPs的中位等效直径因合成条件而异。在模拟胃液中孵育后，观察到SeNPs尺寸增加，而添加模拟肠液后，其尺寸又回到接近初始值。通过使用液相色谱-轨道阱电喷雾电离质谱（LC Orbitrap ESI MS）的非靶向定性分析，确定了酵母提取物中所含的在SeNPs合成中起主要作用的生物分子。使用最先进的质谱技术既可以全面评估导致SeNPs形成的过程，又可以评估它们在胃肠条件下的行为，这对于将其用作新型硒源至关重要。
