College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Hubei, China.
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Hubei, China.
Food Chem. 2021 Jul 15;350:129223. doi: 10.1016/j.foodchem.2021.129223. Epub 2021 Feb 6.
This work investigated selenium (Se) release kinetics and mechanism from exopolysaccharide-selenium nanoparticles (EPS-SeNPs, Se/EPS = 1/20, 1/1 and 4/3) in simulated gastric (SGF) and intestinal fluids (SIF) using kinetics models of Zero order, First order, Higuchi, Hixson-Crowell and Korsmeyer-Peppas. EPS-SeNPs showed an increase in size from 80-125 nm to 250-320 nm and more ambiguous boundary after gastrointestinal digestion. Se/EPS ratio and pH had significant influence on Se release. Se release kinetics from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SGF) followed a classical Fickian diffusion, in contrast to an erosion governed by macromolecular chains relaxation for Se/EPS = 1/20 in SIF. Se release from EPS-SeNPs (Se/EPS = 1/1 and 4/3 in SIF) was well-fitted to Korsmeyer-Peppas model and followed a non-Fickian mechanism controlled by both diffusion and erosion. Additionally, EPS-SeNPs (Se/EPS = 1/20) showed a low Se release after SGF digestion, but a high release after SIF digestion, suggesting its application in controlled release of Se-enriched supplements for Se-deficiency treatment.
本研究采用零级、一级、Higuchi、Hixson-Crowell 和 Korsmeyer-Peppas 动力学模型，考察了在模拟胃液（SGF）和肠液（SIF）中，从胞外多糖-硒纳米颗粒（EPS-SeNPs，Se/EPS=1/20、1/1 和 4/3）中硒的释放动力学和机制。EPS-SeNPs 在胃肠道消化后粒径从 80-125nm 增加到 250-320nm，且边界更加模糊。Se/EPS 比和 pH 值对 Se 释放有显著影响。在 SGF 中，Se/EPS=1/1 和 4/3 的 EPS-SeNPs 的 Se 释放动力学符合经典的菲克扩散，而在 SIF 中，Se/EPS=1/20 的 EPS-SeNPs 的 Se 释放则由大分子链松弛控制的溶蚀过程。在 SIF 中，Se/EPS=1/1 和 4/3 的 EPS-SeNPs 的 Se 释放符合 Korsmeyer-Peppas 模型，遵循由扩散和溶蚀共同控制的非菲克扩散机制。此外，SGF 消化后，EPS-SeNPs（Se/EPS=1/20）的 Se 释放量较低，但 SIF 消化后释放量较高，提示其可用于开发富含 Se 的补充剂的控释，以治疗 Se 缺乏症。
