State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China; Hainan Institute of Wuhan University of Technology, Sanya 572000, China.
Carbohydr Polym. 2023 Jun 15;310:120723. doi: 10.1016/j.carbpol.2023.120723. Epub 2023 Feb 20.
Developing self-healing polysaccharide hydrogels offers a promising strategy for the healing of full-thickness skin wounds. However, the green and facile fabrication of self-healing polysaccharide hydrogel dressings is challenging. Herein, a novel hydrogen-bonded polysaccharide hydrogel consisting only of cationic guar gum (CG) and CuCl was developed by simply mixing CG and Cu solution. A strong enough intermolecular hydrogen bonding could be formed between ipsilateral hydroxyl groups to induce rapid gelation. Benefiting from dynamic and reversible linkages, cationic guar gum-Cu (CG-Cu) hydrogels exhibited self-healing, injectable and self-adaption. The CG-Cu hydrogels possessed favorable mechanical strength (compression strength: 50-89 kPa), excellent biocompatibility (cell viability: >95 %; hemolysis ratio: < 5 %) and satisfying antibacterial ability. In vivo degradation tests showed that the CG-Cu hydrogels could be completely degraded after 21 days. Furthermore, in-situ injected CG-Cu hydrogel dressings could perfectly cover wounds to reduce risk of infection and accelerated full-thickness skin generation. In conclusion, this study may provide a new simple and straightforward strategy to prepare self-healing polysaccharide hydrogels based on hydrogen bonding to expand its application in the field of biomedicine and tissue regeneration.
开发自修复多糖水凝胶为全层皮肤伤口的愈合提供了一种很有前途的策略。然而，绿色且简便地制备自修复多糖水凝胶敷料具有挑战性。在此，通过简单混合 CG 和 Cu 溶液，开发了一种由阳离子瓜尔胶（CG）和 CuCl 组成的新型氢键多糖水凝胶。同侧羟基之间可以形成足够强的分子间氢键，从而诱导快速凝胶化。得益于动态和可逆键合，阳离子瓜尔胶-Cu（CG-Cu）水凝胶表现出自修复、可注射和自适应性。CG-Cu 水凝胶具有良好的机械强度（压缩强度：50-89 kPa）、优异的生物相容性（细胞活力：>95%；溶血率：<5%）和令人满意的抗菌能力。体内降解试验表明，CG-Cu 水凝胶在 21 天后可完全降解。此外，原位注射 CG-Cu 水凝胶敷料可以完美覆盖伤口，降低感染风险并加速全层皮肤生成。总之，该研究可能为基于氢键制备自修复多糖水凝胶提供了一种新的简单策略，以扩展其在生物医学和组织再生领域的应用。
