Li Zhixing, Sun Chenglei, Wang Fan, Xia Zhaofei
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
College of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8654, Japan.
Gels. 2025 May 24;11(6):387. doi: 10.3390/gels11060387.
Alkali-soluble polysaccharides from (APCP) are typically discarded due to poor water solubility and limited bioavailability, despite their β-(1→3)-glucan backbone suggesting potential for functional applications. This study aimed to explore the structural characteristics, gelation behavior, and the capacity of APCP to reduce silver ions. Structural analysis confirmed that APCP is a homogenous β-(1→3)-D-glucan with a molecular weight of 314.2 kDa and a PDI of 1.32. A pH-mediated strategy enabled the formation of stable single-component APCP hydrogel (APCPH) with tunable mechanical strength, high swelling capacity (>590%), and thermal stability. The APCPH further acted as both a reducing and stabilizing matrix for in situ AgNP formation. Notably, the Ag-APCP hydrogel exhibited distinct antibacterial activity, with inhibition zones reaching 5.31 mm against . These findings demonstrate the feasibility of transforming underutilized APCP into multifunctional hydrogel platforms for green nanomaterial synthesis and biomedical applications. Future studies will focus on optimizing AgNP synthesis parameters and evaluating long-term stability and biocompatibility for translational use in antimicrobial therapies.
尽管来自[具体来源未提及]的碱溶性多糖（APCP）具有β-(1→3)-葡聚糖主链，显示出潜在的功能应用潜力，但由于其水溶性差和生物利用度有限，通常会被丢弃。本研究旨在探索APCP的结构特征、凝胶化行为以及还原银离子的能力。结构分析证实，APCP是一种均质的β-(1→3)-D-葡聚糖，分子量为314.2 kDa，多分散指数为1.32。一种pH介导的策略能够形成具有可调机械强度、高溶胀能力（>590%）和热稳定性的稳定单组分APCP水凝胶（APCPH）。APCPH进一步作为原位形成AgNP的还原和稳定基质。值得注意的是，Ag-APCP水凝胶表现出明显的抗菌活性，对[具体菌种未提及]的抑菌圈达到5.31 mm。这些发现证明了将未充分利用的APCP转化为用于绿色纳米材料合成和生物医学应用的多功能水凝胶平台的可行性。未来的研究将集中在优化AgNP合成参数以及评估其在抗菌治疗中的长期稳定性和生物相容性，以实现转化应用。
