Wang Xiaoqin, Li Wenqing, Li Zhenhua, Han Tianjiao, Rong Jinqiao, Fan Jiangbo, Huang Linjuan, Lu Yu, Wang Zhongfu
Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Carbohydr Polym. 2023 Jun 15;310:120728. doi: 10.1016/j.carbpol.2023.120728. Epub 2023 Feb 21.
Human milk glycans are complex carbohydrates, which play a pivotal role in infant health and neonatal development. Maternal secretor status is known to affect free oligosaccharides in milk. Here, the milk N-glycome of secretor (Se+) and nonsecretor (Se-) individuals was qualitatively and quantitatively analyzed by hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry. The total glycosylation, fucosylation, and sialylation of N-glycans was three times higher in the Se+ group compared to the Se- group (p < 0.001) per equal volume of milk. Importantly, 52 out of 63 N-glycans-including the eight most abundant ones-differed greatly between Se+ and Se- individuals (p < 0.05). Moreover, nine N-glycans (H5N3F1, H6N3, H3N5F1, H5N5F1, H5N5F1S1, H5N4F3S1, H6N4F2S1, H6N5F4S1, and H8N7S1) were >10 times more abundant in Se+ milk than in Se- milk. These findings lay a glycomics-basis for designing personalized nutrition supplements for infants.
人乳聚糖是复合碳水化合物，在婴儿健康和新生儿发育中起着关键作用。已知母亲的分泌型状态会影响乳汁中的游离寡糖。在此，通过亲水相互作用色谱-电喷雾电离-串联质谱对分泌型（Se+）和非分泌型（Se-）个体的乳汁N-聚糖进行了定性和定量分析。每等体积乳汁中，Se+组N-聚糖的总糖基化、岩藻糖基化和唾液酸化程度比Se-组高3倍（p<0.001）。重要的是，63种N-聚糖中有52种——包括8种最丰富的N-聚糖——在Se+和Se-个体之间存在显著差异（p<0.05）。此外，9种N-聚糖（H5N3F1、H6N3、H3N5F1、H5N5F1、H5N5F1S1、H5N4F3S1、H6N4F2S1、H6N5F4S1和H8N7S1）在Se+乳汁中的丰度比在Se-乳汁中高10倍以上。这些发现为设计婴儿个性化营养补充剂奠定了糖组学基础。
