State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Anal Chim Acta. 2023 Apr 22;1251:340980. doi: 10.1016/j.aca.2023.340980. Epub 2023 Feb 17.
Developing a simple, economical, and accurate diagnostic method has positive practical significance for the early prevention and intervention of Alzheimer's disease (AD). Herein, combining a closed bipolar electrode (BPE) chip with multicolor electrochemiluminescence (ECL) imaging technology, we constructed a low-voltage driven portable visualized ECL device for the early screening of AD. By introducing parallel resistance, the total resistance of the circuit was greatly reduced. A classical mixture of Ir(ppy) and Ru(bpy) was used as multicolor emitters of the anode with TPrA as the co-reactant. Capture of amyloid-β (Aβ) through antigen-antibody recognition, and signal amplification by electroactive covalent organic frameworks (COF) probe at the cathode of BPE caused the significantly increased faradaic current. The electrical balance of the BPE system resulted in the change of the emission color from green to red at the anode. The ECL-BPE sensor shows good reproducibility and high sensitivity with detection limit of 1 pM by naked eye. The driving voltage is 3.0 V, which means the chip could be driven by two fifth batteries. The visualized ECL-BPE sensor provides a promising point-of-care testing (POCT) tool for the screening of Alzheimer's-related diseases in the early stage.
开发一种简单、经济、准确的诊断方法对阿尔茨海默病（AD）的早期预防和干预具有积极的实际意义。在此，我们结合封闭双极电极（BPE）芯片和多色电化学发光（ECL）成像技术，构建了一种低压驱动的便携式可视化 ECL 设备，用于 AD 的早期筛查。通过引入并联电阻，大大降低了电路的总电阻。经典的 Ir(ppy)和 Ru(bpy)混合物被用作阳极的多色发射器，TPrA 被用作共反应物。通过抗原-抗体识别在 BPE 的阴极捕获淀粉样蛋白-β（Aβ），并通过电活性共价有机框架（COF）探针进行信号放大，导致阳极处的法拉第电流显著增加。BPE 系统的电平衡导致发射颜色从绿色变为红色。ECL-BPE 传感器具有良好的重现性和高灵敏度，通过肉眼检测的检出限低至 1 pM。驱动电压为 3.0 V，这意味着该芯片可以由两节五号电池驱动。可视化 ECL-BPE 传感器为阿尔茨海默病相关疾病的早期筛查提供了一种有前途的即时检测（POCT）工具。
