Cao Danfeng, Martinez Jose G, Anada Risa, Hara Emilio Satoshi, Kamioka Hiroshi, Jager Edwin W H
Sensor and Actuator Systems, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
Advanced Research Center for Oral and Craniofacial Sciences Dental School, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Sci Technol Adv Mater. 2023 Mar 10;24(1):2183710. doi: 10.1080/14686996.2023.2183710. eCollection 2023.
Controlling stem cell behavior at the material interface is crucial for the development of novel technologies in stem cell biology and regenerative medicine. The composition and presentation of bio-factors on a surface strongly influence the activity of stem cells. Herein, we designed an electroactive surface that mimics the initial process of trabecular bone formation, by immobilizing chondrocyte-derived plasma membrane nanofragments (PMNFs) on its surface for rapid mineralization within 2 days. Moreover, the electroactive surface was based on the conducting polymer polypyrrole (PPy), which enabled dynamic control of the presentation of PMNFs on the surface via electrochemical redox switching, further resulting in the formation of bone minerals with different morphologies. Furthermore, bone minerals with contrasting surface morphologies had differential effects on the differentiation of human bone marrow-derived stem cells (hBMSCs) cultured on the surface. Together, this electroactive surface showed multifunctional characteristics, not only allowing dynamic control of PMNF presentation but also promoting the formation of bone minerals with different morphologies within 2 days. This electroactive substrate could be valuable for more precise control of stem cell growth and differentiation, and further development of more suitable microenvironments containing bone apatite for housing a bone marrow stem cell niche, such as biochips/bone-on-chips.
在材料界面控制干细胞行为对于干细胞生物学和再生医学中新技术的发展至关重要。表面生物因子的组成和呈现方式会强烈影响干细胞的活性。在此，我们设计了一种电活性表面，通过在其表面固定软骨细胞衍生的质膜纳米片段（PMNFs）以在2天内实现快速矿化，从而模拟小梁骨形成的初始过程。此外，该电活性表面基于导电聚合物聚吡咯（PPy），它能够通过电化学氧化还原切换对表面PMNFs的呈现进行动态控制，进而导致形成具有不同形态的骨矿物质。此外，具有对比表面形态的骨矿物质对在该表面培养的人骨髓来源干细胞（hBMSCs）的分化有不同影响。总之，这种电活性表面具有多功能特性，不仅允许对PMNF呈现进行动态控制，还能在2天内促进形成具有不同形态的骨矿物质。这种电活性基质对于更精确地控制干细胞生长和分化，以及进一步开发更适合容纳骨髓干细胞龛的含骨磷灰石微环境（如生物芯片/骨片）可能具有重要价值。
