Jirakitticharoen Sirinapha, Wisuitiprot Wudtichai, Jitareerat Pongphen, Wongs-Aree Chalermchai
Division of Postharvest Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Department of Thai Traditional Medicine, Sirindhorn College of Public Health Phitsanulok, Phitsanulok, Thailand.
J Trop Med. 2023 Mar 7;2023:5152506. doi: 10.1155/2023/5152506. eCollection 2023.
The volatiles and antioxidant capacity of essential oils (EOs) extracted from freshly immature and mature leaves of at various hydrodistillation times were investigated. Seven major terpenoids were identified: two monoterpenes, camphor and L-borneol, and five sesquiterpenes, silphiperfol-5-ene, 7-epi-silphiperfol-5-ene, -caryophyllene, -eudesmol, and -eudesmol. The quantity and terpenoid composition of the EOs were impressed by leaf maturity and hydrodistillation times. The yield of EOs from the immature leaves was 1.4 times that of mature leaves, with 73% of the yield acquired within the first 6 hours (hrs) of hydrodistillation. Approximately 97% of camphor and L-borneol, 80% of -caryophyllene, silphiperfolene, and 7-epi-silphiperfolene, 32% of -eudesmol, and 54% -eudesmol were collected in the first 6 hrs of hydrodistillation. More -caryophyllene, -eudesmol, and -eudesmol were found in the mature leaf EOs. The antioxidant capacity of the EOs was proportionally related to their terpenoid contents. The EOs extracted from immature leaves at 0-6 hrs of hydrodistillation demonstrated distinctive antibacterial activity against with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.5 mg/mL and 1 mg/mL, respectively.
研究了从新鲜未成熟和成熟叶片中在不同水蒸馏时间提取的精油（EOs）的挥发性和抗氧化能力。鉴定出七种主要萜类化合物：两种单萜类化合物，樟脑和L-冰片，以及五种倍半萜类化合物，silphiperfol-5-烯、7-表-silphiperfol-5-烯、β-石竹烯、α-桉叶醇和β-桉叶醇。叶片成熟度和水蒸馏时间对EOs的数量和萜类化合物组成有影响。未成熟叶片的EOs产量是成熟叶片的1.4倍，在水蒸馏的前6小时内获得了73%的产量。在水蒸馏的前6小时内收集了约97%的樟脑和L-冰片、80%的β-石竹烯、silphiperfolene和7-表-silphiperfolene、32%的α-桉叶醇和54%的β-桉叶醇。成熟叶片的EOs中发现了更多的β-石竹烯、α-桉叶醇和β-桉叶醇。EOs的抗氧化能力与其萜类化合物含量成正比。在水蒸馏0-6小时从未成熟叶片中提取的EOs对[具体细菌名称未给出]表现出独特的抗菌活性，其最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值分别为0.5mg/mL和1mg/mL。
