Fang Xingqiang, Yang Songjian, Chen Meixi, Sun Ruifan, Zhao Le, Gu Bowen, Zhang Jipan, Huang Deli, Zheng Taikang, Zhao Yuanping, Peng Peng, Zhao Yongju
College of Animal Science and Technology, Southwest University, Chongqing, China.
Chongqing Key Laboratory of Herbivore Science, Chongqing, China.
Anim Biotechnol. 2023 Dec;34(9):4721-4729. doi: 10.1080/10495398.2023.2187406. Epub 2023 Mar 17.
Reproductive traits are essential economic traits in goats. This study aimed to analyze the relationship between single nucleotide polymorphisms (SNPs) within the genes of , , and , and reproductive traits (kidding traits and placental traits) in goats. We used the resequencing data of 150 Dazu Black Goats to perform correlation analysis with the average litter size. We screened thirteen SNPs loci in introns and then used the Sanger method to genotype the remaining 150 Dazu Black Goats. The results showed that a total of six SNPs were screened. Three SNPs related to litter size and live litter size (g.28985790T > G, g.28986352A > G, and g.28987976A > G); one SNP related to total cotyledon area (g.29203243G > A); two SNPs related to placental efficiency (g.30189055G > A and g.30193974C > T); one SNP associated with cotyledon support efficiency (g.30193974C > T). The qPCR results showed that , , and were all highly expressed in the udder, kidney, uterus, and ovary. It indicated that these three candidate genes might affect the reproductive traits, which could be used as candidate markers for reproductive traits in Dazu Black Goats. Moreover, association studies on a large scale are still needed to figure out what effect these SNPs have on reproductive traits.
繁殖性状是山羊重要的经济性状。本研究旨在分析山羊中、和基因内单核苷酸多态性（SNP）与繁殖性状（产羔性状和胎盘性状）之间的关系。我们利用150只大足黑山羊的重测序数据与平均产羔数进行相关性分析。我们在内含子中筛选出13个SNP位点，然后用桑格法对其余150只大足黑山羊进行基因分型。结果共筛选出6个SNP。3个SNP与产羔数和存活产羔数相关（g.28985790T＞G、g.28986352A＞G和g.28987976A＞G）；1个SNP与总子叶面积相关（g.29203243G＞A）；2个SNP与胎盘效率相关（g.30189055G＞A和g.30193974C＞T）；1个SNP与子叶支持效率相关（g.30193974C＞T）。qPCR结果显示，、和在乳腺、肾脏、子宫和卵巢中均高表达。这表明这三个候选基因可能影响繁殖性状，可作为大足黑山羊繁殖性状的候选标记。此外，仍需要大规模的关联研究来弄清楚这些SNP对繁殖性状有何影响。
