• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP).通过环介导等温扩增技术（LAMP）快速灵敏地检测血液中的象内皮嗜性疱疹病毒1型（EEHV1）
J Vet Med Sci. 2019 Mar 30;81(3):504-507. doi: 10.1292/jvms.18-0683. Epub 2019 Jan 30.
2
Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification.通过环介导等温扩增直接检测肝素化血浆中的大象内皮细胞嗜疱疹病毒 1（EEHV1）DNA。
J Vet Med Sci. 2023 Mar 30;85(4):459-462. doi: 10.1292/jvms.22-0578. Epub 2023 Mar 15.
3
Detection of pathogenic elephant endotheliotropic herpesvirus in routine trunk washes from healthy adult Asian elephants (Elephas maximus) by use of a real-time quantitative polymerase chain reaction assay.通过实时定量聚合酶链反应检测法，在健康成年亚洲象（印度象）常规象鼻冲洗液中检测致病性大象内皮嗜性疱疹病毒。
Am J Vet Res. 2010 Aug;71(8):925-33. doi: 10.2460/ajvr.71.8.925.
4
Detection of elephant endotheliotropic herpesvirus infection among healthy Asian elephants (Elephas maximus) in South India.印度南部健康亚洲象（印度象）中大象内皮嗜性疱疹病毒感染的检测
J Wildl Dis. 2014 Apr;50(2):279-87. doi: 10.7589/2012-09-236. Epub 2014 Jan 31.
5
Development of a SYBR Green I-based real-time PCR for detection of elephant endotheliotropic herpesvirus 1 infection in Asian elephants (Elephas maximus).基于 SYBR Green I 的实时 PCR 检测亚洲象（Elephas maximus）中内皮嗜性疱疹病毒 1 感染的建立。
J Virol Methods. 2012 Oct;185(1):160-5. doi: 10.1016/j.jviromet.2012.06.005. Epub 2012 Jun 19.
6
Acute phase protein expression during elephant endotheliotropic herpesvirus-1 viremia in Asian elephants (Elephas maximus).亚洲象（Elephas maximus）感染象内皮嗜性疱疹病毒1型病毒血症期间急性期蛋白的表达
J Zoo Wildl Med. 2013 Sep;44(3):605-12. doi: 10.1638/2012-0174R1.1.
7
Lethal Hemorrhagic Disease and Clinical Illness Associated with Elephant Endotheliotropic Herpesvirus 1 Are Caused by Primary Infection: Implications for the Detection of Diagnostic Proteins.致死性出血性疾病与临床疾病与大象内皮细胞疱疹病毒 1 相关，由原发性感染引起：对诊断蛋白检测的影响。
J Virol. 2020 Jan 17;94(3). doi: 10.1128/JVI.01528-19.
8
Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants.用于检测亚洲象体内大象内皮嗜性疱疹病毒的原位杂交技术的开发。
J Vet Diagn Invest. 2018 Jul;30(4):628-632. doi: 10.1177/1040638718773810. Epub 2018 May 7.
9
Identification of shedders of elephant endotheliotropic herpesviruses among Asian elephants (Elephas maximus) in Switzerland.瑞士亚洲象（印度象）群体中大象内皮嗜性疱疹病毒脱落者的鉴定
PLoS One. 2017 May 3;12(5):e0176891. doi: 10.1371/journal.pone.0176891. eCollection 2017.
10
Subclinical infection of captive Asian elephants (Elephas maximus) in Thailand with elephant endotheliotropic herpesvirus.泰国圈养亚洲象（Elephas maximus）中存在象疱疹病毒亚临床感染。
Arch Virol. 2020 Feb;165(2):397-401. doi: 10.1007/s00705-019-04469-6. Epub 2019 Nov 29.

引用本文的文献

1
A duplex real-time loop-mediated isothermal amplification assay for simultaneous detection of elephant endotheliotropic herpesvirus types 1 and 5.一种用于同时检测1型和5型大象内皮细胞嗜性疱疹病毒的双重实时环介导等温扩增检测方法。
Front Vet Sci. 2025 Aug 26;12:1651182. doi: 10.3389/fvets.2025.1651182. eCollection 2025.
2
Elephant endotheliotropic herpesvirus gB-specific antibody levels in sera of Asian elephants (Elephas maximus) in Japanese zoos.日本动物园亚洲象（Elephas maximus）血清中象疱疹病毒 gB 特异性抗体水平。
J Vet Med Sci. 2024 Dec 1;86(12):1279-1283. doi: 10.1292/jvms.23-0503. Epub 2024 Oct 16.
3
Agarose gel electrophoresis pattern of serum creatine kinase and lactate dehydrogenase isoenzymes in zoo-managed Asian elephants (Elephas maximus).琼脂糖凝胶电泳图谱显示动物园饲养的亚洲象（Elephas maximus）血清肌酸激酶和乳酸脱氢酶同工酶。
J Vet Med Sci. 2023 May 3;85(5):578-583. doi: 10.1292/jvms.22-0550. Epub 2023 Mar 14.
4
Direct detection of elephant endotheliotropic herpesvirus 1 (EEHV1) DNA in heparinized plasma by loop-mediated isothermal amplification.通过环介导等温扩增直接检测肝素化血浆中的大象内皮细胞嗜疱疹病毒 1（EEHV1）DNA。
J Vet Med Sci. 2023 Mar 30;85(4):459-462. doi: 10.1292/jvms.22-0578. Epub 2023 Mar 15.
5
The serum activities of alkaline phosphatase isoenzymes measured using two approved methods in zoo-managed Asian elephants (Elephas maximus).采用两种经批准的方法在动物园管理的亚洲象（Elephas maximus）中测量血清碱性磷酸酶同工酶的活性。
J Vet Med Sci. 2023 Feb 10;85(2):232-235. doi: 10.1292/jvms.22-0440. Epub 2022 Dec 20.
6
Development of Nonstructural Protein-Based Indirect ELISA to Identify Elephant Endotheliotropic Herpesvirus (EEHV) Infection in Asian Elephants ().基于非结构蛋白的间接酶联免疫吸附测定法的开发，用于鉴定亚洲象体内的象内皮嗜性疱疹病毒（EEHV）感染（）。
Animals (Basel). 2022 Jul 7;12(14):1747. doi: 10.3390/ani12141747.

本文引用的文献

1
SUCCESSFUL TREATMENT OF A CLINICAL ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS INFECTION: THE DYNAMICS OF VIRAL LOAD, GENOTYPE ANALYSIS, AND TREATMENT WITH ACYCLOVIR.临床大象嗜内皮性疱疹病毒感染的成功治疗：病毒载量动态、基因型分析及阿昔洛韦治疗
J Zoo Wildl Med. 2017 Dec;48(4):1254-1259. doi: 10.1638/2016-0141R1.1.
2
Subclinical infection of a young captive Asian elephant with elephant endotheliotropic herpesvirus 1.一头圈养的幼年亚洲象感染1型大象嗜内皮疱疹病毒的亚临床感染
Arch Virol. 2018 Feb;163(2):495-500. doi: 10.1007/s00705-017-3628-2. Epub 2017 Nov 1.
3
Surviving and fatal Elephant Endotheliotropic Herpesvirus-1A infections in juvenile Asian elephants - lessons learned and recommendations on anti-herpesviral therapy.幼年亚洲象中大象嗜内皮细胞性疱疹病毒1A感染的存活与致死情况——经验教训及抗疱疹病毒治疗建议
BMC Vet Res. 2016 Aug 27;12(1):178. doi: 10.1186/s12917-016-0806-5.
4
THE IMPACT OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS ON THE CAPTIVE ASIAN ELEPHANT (ELEPHAS MAXIMUS) POPULATION OF THE UNITED KINGDOM AND IRELAND (1995-2013).大象嗜内皮细胞性疱疹病毒对英国和爱尔兰圈养亚洲象（印度象）种群的影响（1995 - 2013年）
J Zoo Wildl Med. 2016 Jun;47(2):405-18. doi: 10.1638/2015-0217.1.
5
Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases.环介导等温扩增法：一种用于动物疾病的创新型基因扩增技术。
Vet World. 2016 May;9(5):465-9. doi: 10.14202/vetworld.2016.465-469. Epub 2016 May 11.
6
CLINICAL INFECTION OF TWO CAPTIVE ASIAN ELEPHANTS (ELEPHAS MAXIMUS) WITH ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS 1B.两头圈养亚洲象（印度象）感染大象嗜内皮细胞性疱疹病毒1B的临床感染情况
J Zoo Wildl Med. 2016 Mar;47(1):319-24. doi: 10.1638/2015-0074.1.
7
Review of Elephant Endotheliotropic Herpesviruses and Acute Hemorrhagic Disease.大象嗜内皮细胞疱疹病毒与急性出血性疾病综述
ILAR J. 2016;56(3):283-96. doi: 10.1093/ilar/ilv041.
8
A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus.一种用于特异性检测抗大象内皮嗜性疱疹病毒IgG抗体的新型抗原捕获酶联免疫吸附测定法。
BMC Vet Res. 2015 Aug 14;11:203. doi: 10.1186/s12917-015-0522-6.
9
Acute phase protein expression during elephant endotheliotropic herpesvirus-1 viremia in Asian elephants (Elephas maximus).亚洲象（Elephas maximus）感染象内皮嗜性疱疹病毒1型病毒血症期间急性期蛋白的表达
J Zoo Wildl Med. 2013 Sep;44(3):605-12. doi: 10.1638/2012-0174R1.1.
10
Complete genome sequences of elephant endotheliotropic herpesviruses 1A and 1B determined directly from fatal cases.直接从致死病例中确定的大象内皮亲和疱疹病毒 1A 和 1B 的完整基因组序列。
J Virol. 2013 Jun;87(12):6700-12. doi: 10.1128/JVI.00655-13. Epub 2013 Apr 3.

通过环介导等温扩增技术（LAMP）快速灵敏地检测血液中的象内皮嗜性疱疹病毒1型（EEHV1）

Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP).

作者信息

Takehana Kazuya, Kinjyo Teruo, Nemoto Manabu, Matsuno Keita

机构信息

Ichihara Elephant Kingdom Zoological Park, Ichihara, Chiba 290-0521, Japan.

Okinawa Zoo and Museum, Okinawa-shi, Okinawa 904-0021, Japan.

出版信息

J Vet Med Sci. 2019 Mar 30;81(3):504-507. doi: 10.1292/jvms.18-0683. Epub 2019 Jan 30.

DOI:10.1292/jvms.18-0683
PMID:30700652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6451909/
Abstract

Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 10 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.

摘要

1型大象内皮嗜性疱疹病毒（EEHV1）是亚洲象（Elephas maximus）急性致命出血性疾病的最重要病原体。我们采用环介导等温扩增技术（LAMP）开发了一种快速简便的检测血液中EEHV1的方法。当用于检测在日本收集的21份临床样本时，EEHV1检测方法正确鉴定出1份阳性和20份阴性临床样本。据观察，当样本中加入包含EEHV1聚合酶基因的合成DNA质粒时，LAMP检测的检测限为10拷贝/微升，比传统PCR高100倍。LAMP检测在EEHV1检测方面的这些优势可能有助于更好地治疗患急性疾病的大象。