通过环介导等温扩增技术（LAMP）快速灵敏地检测血液中的象内皮嗜性疱疹病毒1型（EEHV1）
Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP).
作者信息
Takehana Kazuya, Kinjyo Teruo, Nemoto Manabu, Matsuno Keita
机构信息
Ichihara Elephant Kingdom Zoological Park, Ichihara, Chiba 290-0521, Japan.
Okinawa Zoo and Museum, Okinawa-shi, Okinawa 904-0021, Japan.
出版信息
J Vet Med Sci. 2019 Mar 30;81(3):504-507. doi: 10.1292/jvms.18-0683. Epub 2019 Jan 30.
Abstract
Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 10 copies/µl and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.
摘要
1型大象内皮嗜性疱疹病毒（EEHV1）是亚洲象（Elephas maximus）急性致命出血性疾病的最重要病原体。我们采用环介导等温扩增技术（LAMP）开发了一种快速简便的检测血液中EEHV1的方法。当用于检测在日本收集的21份临床样本时，EEHV1检测方法正确鉴定出1份阳性和20份阴性临床样本。据观察，当样本中加入包含EEHV1聚合酶基因的合成DNA质粒时，LAMP检测的检测限为10拷贝/微升，比传统PCR高100倍。LAMP检测在EEHV1检测方面的这些优势可能有助于更好地治疗患急性疾病的大象。
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