Institute for Chemical Research, Kyoto University.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology.
Microbes Environ. 2023;38(1). doi: 10.1264/jsme2.ME22102.
We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15 kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50 ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.
我们在此提出了一种快速简便的环境微生物样品 DNA 和 RNA 共提取方法。它结合了珠磨和酚氯仿相分离，然后使用 Qiagen AllPrep DNA/RNA mini 试剂盒分离和纯化 DNA 和 RNA。我们的方法处理时间约为 3 小时，可同时从过滤的湖细菌浮游生物样本中提取高质量的 DNA（峰大小> 10-15kb）和 RNA（RNA 完整性数> 6）。该方法也适用于低生物量样品（预期 DNA 或 RNA 产量<50ng）和真核微生物样品，为更通用的生态基因组应用提供了一种简单的选择。
