晋江河口悬浮颗粒物对铜绿微囊藻生理生化特性的影响。
Effects of suspended particles in the Jinjiang River Estuary on the physiological and biochemical characteristics of Microcystis flos-aquae.
机构信息
Department of Environmental Science and Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, 361021, Fujian, China.
Institute of Environment and Resources Technology, Huaqiao University, Xiamen, 361021, China.
出版信息
Environ Sci Pollut Res Int. 2023 Apr;30(19):56687-56699. doi: 10.1007/s11356-023-26367-w. Epub 2023 Mar 16.
The effects of different concentrations (100, 150, 200, 250 mg/L) and different particle sizes (0-75 μm, 75-120 μm, 120-150 μm, 150-500 μm) on the soluble protein content, superoxide dismutase (SOD) and catalase (CAT) activity, malondialdehyde (MDA) content, chlorophyll a (Chla) content, and photosynthetic parameters of Microcystis flos-aquae were studied, and the mechanism of the effect of suspended particulate matter on the physiology and biochemistry of Microcystis flos-aquae was discussed. The results showed that the soluble protein content of Microcystis flos-aquae did not change noticeably after being stressed by suspended particles of different concentrations/diameters. The SOD activity of Microcystis flos-aquae first increased and then decreased with increasing suspended particulate matter concentrations. The SOD activity of Microcystis flos-aquae reached 28.03 U/mL when the concentration of suspended particulate matter was 100 mg/L. The CAT activity of Microcystis flos-aquae increased with increasing concentrations of suspended particles and reached a maximum value of 12.45 U/mg prot in the 250 mg/L concentration group, showing a certain dose effect. Small particles had a more significant effect on SOD, CAT, and MDA in Microcystis flos-aquae than large particles. The larger the concentration was and the smaller the particle size was, the stronger the attenuation of light and the lower the content of Chla. Both the maximum quantum yield of PSII (Fv/Fm) and the potential photosynthetic activity of PSII (Fv/F) of Microcystis flos-aquae increased at first and then decreased under different concentrations/sizes of suspended particles. The relative electron transfer rate gradually returned to a normal level over time. There was no significant difference in the initial slope (α) value between the treatment group and the control group, and the maximum photo synthetic rate (ETRmax) and the semilight saturation (I) decreased.
研究了不同浓度（100、150、200、250 mg/L）和不同粒径（0-75 μm、75-120 μm、120-150 μm、150-500 μm）对铜绿微囊藻可溶性蛋白含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、丙二醛（MDA）含量、叶绿素 a（Chla）含量和光合参数的影响，并探讨了悬浮颗粒物对铜绿微囊藻生理生化的影响机制。结果表明，不同浓度/粒径的悬浮颗粒物胁迫下铜绿微囊藻可溶性蛋白含量变化不明显。铜绿微囊藻 SOD 活性随悬浮颗粒物浓度的增加先升高后降低，当悬浮颗粒物浓度为 100 mg/L 时，SOD 活性达到 28.03 U/mL。铜绿微囊藻 CAT 活性随悬浮颗粒物浓度的增加而升高，在 250 mg/L 浓度组达到 12.45 U/mg prot，表现出一定的剂量效应。小颗粒对铜绿微囊藻 SOD、CAT 和 MDA 的影响大于大颗粒。浓度越大，粒径越小，光衰减越强，Chla 含量越低。不同浓度/粒径的悬浮颗粒物作用下，铜绿微囊藻 PSII 最大光化学量子产量（Fv/Fm）和潜在光化学活性（Fv/F）先升高后降低，相对电子传递速率（rETR）随时间逐渐恢复正常水平。处理组与对照组之间初始斜率（α）值无显著差异，最大光合速率（ETRmax）和半饱和光强（I）降低。
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