Wang Meixian, Zhang Yuxuan, Guo Peiyong
Department of Environmental Science and Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China; Institute of Environmental and Resources Technology, Huaqiao University, Xiamen 361021, China.
Department of Environmental Science and Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China; Institute of Environmental and Resources Technology, Huaqiao University, Xiamen 361021, China.
Aquat Toxicol. 2017 May;186:67-76. doi: 10.1016/j.aquatox.2017.02.022. Epub 2017 Feb 22.
Florfenicol (FF) and thiamphenicol (TAP) are two typical pharmaceuticals used widely as therapeutica antibiotic agents in aquaculture. However, little is known about the potential adverse effects of these two antibiotics on non-target organisms in the aquatic ecosystem. In this study we investigated the effects of FF and TAP on photosynthesis and the antioxidant system of the cyanobacteria Microcystis flos-aquae. Over a concentration range of 0.001-1μg/L, the results showed that both FF and TAP significantly increased the chlorophyll a content of M. flos-aquae, while the superoxide dismutase (SOD) activity, catalase (CAT) activity and the levels of malondialdehyde (MDA) changed slightly. In contrast, the chlorophyll a content of M. flos-aqua was significantly inhibited (p<0.01) at high concentrations (>1μg/L) of FF and TAP, reaching a 46% inhibition level at 50μg/L FF and 56% inhibition at 100μg/L TAP. At the same time, the activities of SOD and CAT along with MDA content also increased significantly (p<0.01), indicating that the high concentrations of both FF and TAP led to oxidative stress in the algae. In addition, the M. flos-aquae fluorescence parameters (Fv/Fm, Fv/Fo, alpha, ETRmax and Ik) increased with increasing concentration of both FF and TAP, which may be the result of the increasing photoprotection capacity.
氟苯尼考（FF）和甲砜霉素（TAP）是水产养殖中广泛用作治疗性抗生素的两种典型药物。然而，关于这两种抗生素对水生生态系统中非靶标生物的潜在不利影响知之甚少。在本研究中，我们调查了FF和TAP对水华微囊藻光合作用和抗氧化系统的影响。在0.001 - 1μg/L的浓度范围内，结果表明FF和TAP均显著增加了水华微囊藻的叶绿素a含量，而超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性和丙二醛（MDA）水平变化不大。相反，在FF和TAP的高浓度（>1μg/L）下，水华微囊藻的叶绿素a含量受到显著抑制（p<0.01），在50μg/L FF时抑制水平达到46%，在100μg/L TAP时抑制水平达到56%。同时，SOD和CAT的活性以及MDA含量也显著增加（p<0.01），表明高浓度的FF和TAP均导致藻类产生氧化应激。此外，水华微囊藻的荧光参数（Fv/Fm、Fv/Fo、α、ETRmax和Ik）随着FF和TAP浓度的增加而增加，这可能是光保护能力增强的结果。
