Demiroz Akbulut Tugce, Aydin Kose Fadime, Demirci Betul, Baykan Sura
Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35040, Izmir, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Izmir Katip Celebi University, 35620, Izmir, Turkey.
Chem Biodivers. 2023 Apr;20(4):e202201188. doi: 10.1002/cbdv.202201188. Epub 2023 Mar 24.
Marrubium vulgare L. (Lamiaceae) is used for respiratory and gastrointestinal system disorders in folk medicine. According to European Pharmacopoeia criteria, standardization of the plant is defined by its marrubiin content. In present study, phenolics, marrubiin and essential oil compositions of M. vulgare from different locations in Turkey were analyzed quantitatively by UPLC, GC and GC/MS. Besides, their cytotoxic potentials were evaluated. In the samples, forsythoside B (77-400 mg/100 g dw), arenarioside (forsythoside F) (0-241 mg/100 g dw), verbascoside (acteoside) (171-416 mg/100 g dw) and apigenin-7-O-glucoside (0-17 mg/100 g dw) were determined in different ranges. Marrubiin contents (0.58-1.46 %) of some samples were two times higher than European Pharmacopoeia standards (0.7 %). β-Caryophyllene (7.24-20.34 %), (Z)-β-farnesene (1.58-34.85 %), germacrene D (9.8-13.37 %), bicyclogermacrene (1.71-8.63 %) and β-bisabolene (0-16.68 %) were detected as major compounds in essential oils. The sample from the west of Aegean Region showed cytotoxicity against human neuroblastoma (SH-SY5Y) cell lines (IC : 59.80 μg/mL) although it has no effect on non-cancerous NIH-3T3cell lines. This is the first report on phenolic profiles of M. vulgare populations from Turkey. Their potential as marrubiin source for pharmaceutical industry should be considered.
欧夏至草（唇形科）在民间医学中用于治疗呼吸系统和胃肠道系统疾病。根据欧洲药典标准，该植物的标准化以其夏至草素含量来定义。在本研究中，采用超高效液相色谱（UPLC）、气相色谱（GC）和气相色谱-质谱联用（GC/MS）对土耳其不同地区的欧夏至草中的酚类、夏至草素和精油成分进行了定量分析。此外，还评估了它们的细胞毒性潜力。在样品中，连翘酯苷B（77 - 400毫克/100克干重）、砂生苷（连翘酯苷F）（0 - 241毫克/100克干重）、毛蕊花糖苷（阿克苷）（171 - 416毫克/100克干重）和芹菜素-7-O-葡萄糖苷（0 - 17毫克/100克干重）在不同范围内被检测到。一些样品的夏至草素含量（0.58 - 1.46%）比欧洲药典标准（0.7%）高出两倍。β-石竹烯（7.24 - 20.34%）、（Z）-β-法呢烯（1.58 - 34.85%）、吉马烯D（9.8 - 13.37%）、双环吉马烯（1.71 - 8.63%）和β-红没药烯（0 - 16.68%）被检测为精油中的主要成分。爱琴海地区西部的样品对人神经母细胞瘤（SH-SY5Y）细胞系显示出细胞毒性（IC₅₀：59.80微克/毫升），尽管它对非癌性的NIH-3T3细胞系没有影响。这是关于土耳其欧夏至草种群酚类特征的首次报道。应考虑它们作为制药工业中夏至草素来源的潜力。
