荩草素、荩草提取物和精油对致病性皮肤癣菌的抗氧化和抗真菌活性。

Antioxidant and antifungal activities of marrubiin, extracts and essential oil from Marrubium vulgare L. against pathogenic dermatophyte strains.

机构信息

Faculty of Sciences of Tunisia, University Tunis El Manar, 2092 Tunis, Tunisia.

出版信息

J Mycol Med. 2020 Apr;30(1):100927. doi: 10.1016/j.mycmed.2020.100927. Epub 2020 Jan 11.

DOI:10.1016/j.mycmed.2020.100927

PMID:31983544

Abstract

OBJECTIVE

Medicinal plants extracts and plant-derived compounds are one of the natural sources for discovering new antifungal agents, the objectives of this work were to investigate for the first time the antidermatophytic, antipathogenic activities of methanol, acetone extracts, and essential oil of Marrubium vulgare L. grown in Tunisia and its active compound marrubiin on pathogenic for animals and humans, such as some dermatophytes and pathogenic for plants, and to evaluate antioxidant activities of different extracts with consideration to their chemical compositions.

MATERIAL AND METHODS

Acetone and methanol extracts were evaluated by HPLC, the essential oil was also analyzed by GC/MS. PCL assay was used to determine the antioxidant activity.

RESULTS

Results showed that methanol and acetone extracts exhibited a significant antioxidant activity (261.41 and 272.90μmol TE/g respectively), while the lowest one was observed in the case of marrubiin and essential oil. The antifungal activity of different extracts, marrubiin and essential oil at two concentrations (20 and 100μg/mL) were screened against the dermatophytes fungi Microsporum gypseum, Microsporum canis, Arthroderma cajetani, Trichophyton mentagrophytes, Trichophyton tonsurans, Epidermophyton floccosum and against two fungi strains (Botrytis cinerea, Pythium ultimum). Among tested extracts, marrubiin at 100μg/mL showed about 50% inhibition for T. mentagrophytes and E. floccosum. The anti-phytopathogenic activity was also carried out, only marrubiin had in activity against B. cinerea at the highest dose (32.40%), while methanol extract of M.vulgare and marrubiin are able to increase the mycelial growth of P. ultimum at the highest concentration (45.15 and 40.30% respectively).

CONCLUSION

In our study, we conclude that M.vulgare and marrubiin can be used as natural antioxidants and antifungal agent for treatment of skin dermatophyte infections.

摘要

目的

药用植物提取物和植物衍生化合物是发现新型抗真菌药物的天然来源之一。本研究的目的是首次研究突尼斯生长的马郁兰（Marrubium vulgare L.）的甲醇、丙酮提取物和精油及其活性化合物马郁兰素对动物和人类致病的抗真菌、抗植物病原真菌的活性，并考虑其化学成分评估不同提取物的抗氧化活性。

材料与方法

通过高效液相色谱法（HPLC）评估丙酮和甲醇提取物，用气相色谱/质谱法（GC/MS）分析精油。PCL 测定法用于测定抗氧化活性。

结果

结果表明，甲醇和丙酮提取物表现出显著的抗氧化活性（分别为 261.41 和 272.90μmol TE/g），而马郁兰素和精油的活性最低。不同提取物、马郁兰素和精油在两个浓度（20 和 100μg/mL）下对皮肤癣菌真菌石膏样小孢子菌、犬小孢子菌、卡耶特氏毛癣菌、须癣毛癣菌、疣状毛癣菌、絮状表皮癣菌和两种真菌菌株（灰葡萄孢、腐霉）的抗真菌活性进行了筛选。在所测试的提取物中，马郁兰素在 100μg/mL 时对 T. mentagrophytes 和 E. floccosum 表现出约 50%的抑制作用。还进行了抗植物病原活性的研究，只有马郁兰素在最高剂量（32.40%）时对 B. cinerea 具有活性，而马郁兰素和马郁兰素的甲醇提取物能够在最高浓度（分别为 45.15%和 40.30%）下增加腐霉的菌丝生长。