School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
Institute of Bioresources and Sustainable Development, Institute of Bioresources and Sustainable DevelopmentDepartment of Biotechnology, Govt. of India, Imphal, India.
J Sep Sci. 2023 May;46(10):e2200872. doi: 10.1002/jssc.202200872. Epub 2023 Mar 27.
Trachyspermum ammi (L.) Sprague (Apiaceae), commonly known as "Ajwain" is distributed throughout India. Ajwain fruits contain fiber, carbohydrates, phenolic acids, flavonoids, and tannins. The fruits also yield a small amount of essential oil, with Thymol as the principal constituent. Ajwain has various pharmacological activities like anti-leishmanial, antimicrobial, cytotoxic, antispasmodic, nematocidal, and anthelmintic. The fruits are of high therapeutic value; thus, it becomes quite essential to evaluate the quality of Trachyspermum ammi (L.) Sprague to authenticate and ensure its therapeutic and nutritional properties. The ethyl acetate fraction of Trachyspermum ammi (L.) Sprague fruits exhibited the highest total phenolic and flavonoid content values of 149.55 ± 1.19 mg rutin equivalent and 682.85 ± 3.68 mg gallic acid equivalent, respectively. Metabolite profiling of the ethyl acetate fraction using ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry analysis resulted in identifying 19 phytomolecules. A validated high-performance thin-layer chromatography method was developed to quantify standard phytomolecules in the ethyl acetate fraction. The highest and lowest percentages of phytomarker were found to be caffeic acid (5.51% ± 0.16% w/w) and gallic acid (1.29% ± 0.09% w/w), respectively. This validated rapid, accurate, and precise method for standardization of Trachyspermum ammi (L.) Sprague will be beneficial for its quality evaluation as well as the derived products.
莳萝（Apiaceae），俗称“孜然”，分布于印度各地。孜然果实含有纤维、碳水化合物、酚酸、类黄酮和单宁。果实还产生少量精油，以百里香酚为主成分。孜然具有多种药理活性，如抗利什曼原虫、抗菌、细胞毒性、抗痉挛、杀线虫和驱虫作用。果实具有很高的治疗价值；因此，评估莳萝（L.）斯普拉格的质量以验证并确保其治疗和营养特性变得非常重要。莳萝（L.）斯普拉格果实的乙酸乙酯部分表现出最高的总酚和类黄酮含量值，分别为 149.55 ± 1.19 mg 芦丁当量和 682.85 ± 3.68 mg 没食子酸当量。使用超高效液相色谱-四极杆飞行时间串联质谱分析对乙酸乙酯部分进行代谢物分析，结果鉴定出 19 种植物分子。开发了一种经过验证的高效薄层色谱法来定量乙酸乙酯部分中的标准植物分子。发现最高和最低的植物标志物百分比分别为咖啡酸（5.51%±0.16%w/w）和没食子酸（1.29%±0.09%w/w）。这种经过验证的快速、准确和精确的莳萝（L.）斯普拉格标准化方法将有利于其质量评估以及衍生产品。
