Division of Laboratory Animal Monitoring, National Institutes for Food and Drug Control, Beijing, People's Republic of China.
China National Rodent Laboratory Animal Resources Center, Beijing, People's Republic of China.
Emerg Microbes Infect. 2023 Dec;12(1):e2192822. doi: 10.1080/22221751.2023.2192822.
The current study was designed to comparatively analyse the reactions of different mouse strains in response to vaccine, with attempt to further provide a reference for aP vaccine evaluation. NIH mice, ICR mice, and BALB/c mice adopted from different pharmacopoeias and studies were utilized to measure the immune protection and immunogenicity of the same batch of aP vaccine according to the from some Asian pharmacopoeias and the method from European Pharmacopoeia. Based on our results, the aP vaccine detected by NIH mice had the best potency. So the NIH mice were more suitable for detecting the immune protection of aP vaccine by the MICA method. Given that the levels of PT-IgG and FHA-IgG antibodies in ICR mice were the highest, and the levels of Th1 and Th2 cells were significantly increased ( < .01), it was more suitable for the detection of immunogenicity of aP vaccine by PSPT method. Spleen lymphocytes were stimulated by PT and FHA. And the levels of IL-4 in ICR mice and NIH mice were significantly increased, so were the levels of IL-17, IL-23, IL-27, and TNF-α in BALB/c mice. NIH mice have stronger adaptive immunity and the weakest inflammatory response, and ICR mice have enhanced adaptive immunity and inflammatory responses, both of which can be thereby used for evaluation by different pharmacopoeia methods. NIH was more suitable for the MICA method of Chinese Pharmacopoeia, and ICR for the PSPT method of European Pharmacopoeia.
本研究旨在比较分析不同品系小鼠对疫苗的反应，以期为 aP 疫苗评价提供参考。本研究采用来自不同药典和研究的 NIH 小鼠、ICR 小鼠和 BALB/c 小鼠，根据亚洲药典和欧洲药典的方法，对同一批 aP 疫苗的免疫保护和免疫原性进行检测。结果显示，NIH 小鼠检测的 aP 疫苗效价最好，因此更适合采用 MICA 法检测 aP 疫苗的免疫保护作用。ICR 小鼠的 PT-IgG 和 FHA-IgG 抗体水平最高，Th1 和 Th2 细胞水平显著升高（ < 0.01），更适合采用 PSPT 法检测 aP 疫苗的免疫原性。PT 和 FHA 刺激脾淋巴细胞，ICR 小鼠和 NIH 小鼠的 IL-4 水平显著升高，BALB/c 小鼠的 IL-17、IL-23、IL-27 和 TNF-α水平也显著升高。NIH 小鼠适应性免疫较强，炎症反应较弱，ICR 小鼠适应性免疫增强，炎症反应增强，均可采用不同的药典方法进行评价。NIH 小鼠更适合中国药典的 MICA 方法，ICR 小鼠更适合欧洲药典的 PSPT 方法。
