Doerschuk C M, Downey G P, Doherty D E, English D, Gie R P, Ohgami M, Worthen G S, Henson P M, Hogg J C
University of British Columbia Pulmonary Research Laboratory, Vancouver, Canada.
J Appl Physiol (1985). 1990 May;68(5):1956-61. doi: 10.1152/jappl.1990.68.5.1956.
These studies compare the behavior of radiolabeled neutrophils, monocytes, lymphocytes, and platelets during their first pass through the pulmonary circulation after a central venous injection and their distribution within the circulation 10 min later. Their first pass through the pulmonary circulation was compared with erythrocytes (RBCs) using the indicator-dilution technique, and their recovery within the circulation of the lung and other organs was determined at 10 min by counting the radioisotopes in each organ. The extraction of each cell relative to RBCs during the first pass through the lung correlated with cell size in that the neutrophils (volume 107-140 fl) showed 97.6 +/- 0.6% extraction, monocytes (volume 80-105 fl) showed 91.4 +/- 1.7% extraction, lymphocytes (volume 36-75 fl) showed 80.1 +/- 4.4% extraction, and platelets (volume 4-7 fl) showed 33.1 +/- 3.9% extraction. After 10 min of circulation, the proportion of injected cells remaining in the lung was similar for neutrophils and monocytes (27.4 +/- 1.8 vs. 31.4 +/- 1.6%) but lower for lymphocytes (18.6 +/- 2.9%) and platelets (3.1 +/- 0.5%). All of the leukocytes were found to have a substantial marginated pool within the lung, whereas the platelets did not. The exchange between the circulating and marginated pools of leukocytes in the lung was related to blood velocity, with the least retention occurring in lung regions with shortest RBC transit times. We conclude that cell size is a major factor determining the time that cells will be delayed by the pulmonary microvasculature.(ABSTRACT TRUNCATED AT 250 WORDS)
这些研究比较了放射性标记的中性粒细胞、单核细胞、淋巴细胞和血小板在经中心静脉注射后首次通过肺循环期间的行为，以及10分钟后它们在循环系统中的分布情况。使用指示剂稀释技术将它们首次通过肺循环的情况与红细胞（RBCs）进行比较，并在10分钟时通过计数每个器官中的放射性同位素来确定它们在肺和其他器官循环中的回收率。在首次通过肺部时，每个细胞相对于红细胞的摄取率与细胞大小相关，即中性粒细胞（体积107 - 140飞升）的摄取率为97.6 +/- 0.6%，单核细胞（体积80 - 105飞升）为91.4 +/- 1.7%，淋巴细胞（体积36 - 75飞升）为80.1 +/- 4.4%，血小板（体积4 - 7飞升）为33.1 +/- 3.9%。循环10分钟后，留在肺内的注入细胞比例，中性粒细胞和单核细胞相似（27.4 +/- 1.8%对31.4 +/- 1.6%），但淋巴细胞（18.6 +/- 2.9%）和血小板（3.1 +/- 0.5%）较低。发现所有白细胞在肺内都有一个大量的边缘池，而血小板没有。肺内白细胞循环池和边缘池之间的交换与血流速度有关，在红细胞通过时间最短的肺区域中滞留最少。我们得出结论，细胞大小是决定细胞被肺微血管延迟时间的主要因素。（摘要截短于250字）
