UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia, 56000 Kuala Lumpur, Malaysia.
Iran J Immunol. 2023 Mar 14;20(1):83-91. doi: 10.22034/iji.2023.92600.2171.
Cancer testis antigens (CTAs) are a class of immune-stimulating antigens often overexpressed in many types of cancers. The usage of the CTAs as immunotherapy targets have been widely investigated in different cancers including melanoma, hematological malignancies, and colorectal cancer. Studies have indicated that the epigenetic regulation of the CTAs such as the methylation status may affect the expression of the CTAs. However, the report on the methylation status of the CTAs is conflicting. The general methylation profile of the CTAs, especially in colorectal cancer, is still elusive.
To determine the methylation profile of the selected CTAs in our colorectal cancer patients.
A total of 54 pairs of colorectal cancer samples were subjected to DNA methylation profiling using the Infinium Human Methylation 450K bead chip.
We found that most of the CTAs were hypomethylated, and CCNA1 and TMEM108 genes were among the few CTAs that were hypermethylated.
Overall, our brief report has managed to show the overall methylation profile in over the 200 CTAs in colorectal cancer and this could be used for further refining any immunotherapy targets.
癌症睾丸抗原（CTAs）是一类在许多类型的癌症中经常过度表达的免疫刺激抗原。在黑色素瘤、血液恶性肿瘤和结直肠癌等不同癌症中，已经广泛研究了将 CTA 用作免疫治疗靶点。研究表明，CTA 的表观遗传调控，如甲基化状态，可能会影响 CTA 的表达。然而，关于 CTA 的甲基化状态的报告存在矛盾。CTA 的一般甲基化谱，特别是在结直肠癌中，仍然难以捉摸。
确定我们的结直肠癌患者中选定的 CTA 的甲基化谱。
使用 Infinium Human Methylation 450K 珠芯片对 54 对结直肠癌样本进行 DNA 甲基化分析。
我们发现大多数 CTA 呈低甲基化，CCNA1 和 TMEM108 基因是少数呈高甲基化的 CTA 之一。
总的来说，我们的简要报告成功地展示了结直肠癌中超过 200 个 CTA 的总体甲基化谱，这可以用于进一步细化任何免疫治疗靶点。
