College of Life and Health Sciences, Northeastern University, Shenyang 110169, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
Anal Chim Acta. 2018 Jan 25;999:13-26. doi: 10.1016/j.aca.2017.10.026. Epub 2017 Oct 27.
Inner filter effect (IFE) was previously considered as an error in fluorescence measurement. In recent years, it has been developed as an important non-irradiation energy conversion model of spectroscopic technique and found wide applications in the fields of chemical sensing and biosensing. In comparison with traditional techniques based on forster resonance energy transfer (FRET), the IFE-based fluorescent approach is more flexible and straightforward without the link of absorber with fluorescer. The present review for the first time introduces the state of the art in the progress of the IFE-based fluorescent sensing systems, including sensing strategy, essential conditions, materials option, and their applications for the detection of various target analytes, e.g., ionic species, small molecules, and macromolecules. In addition, the benefits and limitations of the IFE-based fluorescent sensing systems are also critically discussed and highlighted.
内滤效应（IFE）以前被认为是荧光测量中的一种误差。近年来，它已发展成为光谱技术中一种重要的非辐射能量转换模型，并在化学传感和生物传感领域得到了广泛应用。与基于Förster 共振能量转移（FRET）的传统技术相比，基于 IFE 的荧光方法更加灵活和直接，无需将吸收体与荧光体连接。本文首次介绍了基于 IFE 的荧光传感系统的最新进展，包括传感策略、基本条件、材料选择及其在检测各种目标分析物（如离子物种、小分子和大分子）中的应用。此外，还对基于 IFE 的荧光传感系统的优缺点进行了批判性讨论和强调。
