检测不同类型犬舍犬只不同解剖部位的支原体种属。
Detection of Mycoplasma species at various anatomical sites of dogs from different types of kennels.
机构信息
Departamento de Saúde Coletiva Veterinária E Saúde Pública, Universidade Federal Fluminense, Niterói, Brazil.
Universidade Estadual Do Rio de Janeiro E Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
出版信息
Braz J Microbiol. 2023 Jun;54(2):1251-1255. doi: 10.1007/s42770-023-00947-x. Epub 2023 Mar 18.
This work aimed to detect Mycoplasma cynos, M. canis, M. edwardii, and M. molare in different types of kennels, in addition to evaluating their distribution in different colonization sites. The dogs belonged to different kennels from armed forces (n = 3), shelters (n = 3), and commercial purposes (n = 2). Samples of the oropharynx, genital mucosa, and ear canal were collected from each dog (n = 98), totaling 294 samples. Aliquots were submitted to isolation and the samples confirmed as Mycoplasma spp. were subjected to conventional PCR for M. canis and multiplex PCR for M. edwardii, M. molare, and M. cynos detection. Of the 98 dogs studied, 63.3% (62) were positive in at least one anatomical site evaluated for Mycoplasma spp. Among the 111 anatomical sites positive for Mycoplasma spp., M. canis, M. edwardii, and M. molare were detected in 29.7% (33/111), 40.5% (45/111), and 2.70% (3/111), respectively. No animal was positive for M. cynos.
本研究旨在检测不同类型犬舍中的犬嗜血支原体、犬支原体、爱德华氏支原体和摩拉氏支原体，并评估它们在不同定植部位的分布情况。这些犬来自于武装部队犬舍（n=3）、收容所犬舍（n=3）和商业犬舍（n=2）。从每只犬的口咽、生殖道和耳道采集样本（n=98），共计 294 个样本。将部分样本进行分离，将确认为支原体属的样本进行常规 PCR 检测犬支原体，以及多重 PCR 检测爱德华氏支原体、摩拉氏支原体和犬嗜血支原体。在所研究的 98 只犬中，至少有一个解剖部位检测到支原体属阳性的占 63.3%（62 只）。在 111 个检测到支原体属阳性的解剖部位中，分别有 29.7%（33/111）、40.5%（45/111）和 2.70%（3/111）检测到犬支原体、爱德华氏支原体和摩拉氏支原体。没有动物检测到犬嗜血支原体。
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