诱导小鼠房室传导系统细胞消融的方案。

Protocol for inducing cellular ablation in the mouse atrioventricular conduction system.

机构信息

Department of Internal Medicine, Cardiology Division, UT Southwestern Medical Center, Dallas, TX 75390, USA.

Department of Internal Medicine, Cardiology Division, UT Southwestern Medical Center, Dallas, TX 75390, USA; College of Medicine, University of the Philippines Manila, Metro Manila 1000, Philippines.

出版信息

STAR Protoc. 2023 Mar 17;4(1):102145. doi: 10.1016/j.xpro.2023.102145. Epub 2023 Mar 11.

DOI:10.1016/j.xpro.2023.102145

PMID:36933220

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10024233/

Abstract

Damage to the atrioventricular conduction system (AVCS), the main electrical connection between the atrial and ventricular chambers, can result in a variety of cardiac conduction disorders. Here, we provide a protocol for selective damage of the mouse AVCS to study its response during injury. We describe tamoxifen-induced cellular ablation, detection of AV block through electrocardiography, and quantification of histological and immunofluorescence markers to analyze the AVCS. This protocol can be used to study mechanisms associated with AVCS injury repair and regeneration. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021)..

摘要

房室传导系统（AVCS）损伤会导致各种心脏传导障碍，AVCS 是心房和心室之间的主要电连接。在这里，我们提供了一种选择性损伤小鼠 AVCS 的方案，以研究其在损伤过程中的反应。我们描述了他莫昔芬诱导的细胞消融、心电图检测房室传导阻滞以及组织学和免疫荧光标记物的定量分析，以分析 AVCS。该方案可用于研究与 AVCS 损伤修复和再生相关的机制。有关该方案使用和执行的完整详细信息，请参阅 Wang 等人。（2021 年）。