Construction of a robust turn-on fluorescence NIR sensor for rapid detection and imaging of ONOO in inflammatory models.

Peroxynitrite (OONO) is closely related to the occurrence and development of health and inflammatory diseases. The physiological and pathological results of OONO are related to the local concentration of ONOO. Therefore, to develop of a simple, rapid and reliable OONO detection tool is badly needed. In this work, we developed a small-molecule near-infrared (NIR) turn-on fluorescence sensor (NN1), harnessing a well-known response group phenylboronic acid response toward OONO. It shows high detection sensitivity and yields a ratio (I/I) fluorescence enhancement (∼280-fold). In addition, NN1 can be effectively used to detect endogenous and exogenous ONOO in living inflammatory cells. Notably, NN1 can be applied to OONO imaging analysis in drug-induced inflammatory mice model with satisfactory results. Therefore, NN1 is a robust molecular biological tool, which has a good prospect in the study of ONOO and the occurrence and development of inflammatory diseases.