Department of Stomatology, The 3rd Xiangya Hospital, Central South University, Changsha 410013, PR China.
Chongqing Key Laboratory for Pharmaceutical Metabolism Research, The Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmacy, Chongqing Medical University, Chongqing 400016, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 15;291:122381. doi: 10.1016/j.saa.2023.122381. Epub 2023 Jan 18.
Changed levels of intracellular peroxynitrite anion (ONOO) are closely related to the occurrence and development of inflammation. Specific imaging of ONOO at sites of inflammation can be of great significance not only for inflammation diagnosis but also for obtaining a deeper understanding of the role of ONOO in inflammation. Therefore, there is an urgent need for constructing some reliable tools to study the relationship between ONOO and inflammation in biosystems. In this work, we developed a robust high selectivity fluorescence turn-on nanoprobe (Rhb-ONOO) for inflammation-targeted imaging of ONOO. The Rhb-ONOO was obtained by self-assembly of amphiphilic Rhb-ONOO, which was constructed by the condensation reaction of the hydrophobic, ONOO-response and deep red-emitting fluorophore (Rhb) with hydrophilic biopolymer glycol chitosan (GC). Rhb-ONOO showed rapid response towards ONOO during 60 s, high sensitivity with 19-fold enhancement of fluorescence intensity ratio (I/I), and excellent selectivity towards ONOO over other analytes as well as a good linear relationship was observed between the I/I and the ONOO concentration range 0-1 μM, with an excellent limit of detection (LOD) of 33 nM. Impressively, it was successfully employed Rhb-ONOO for ONOO imaging in living inflammatory cells and drug-induced inflammatory mice, illustrating nanoprobe Rhb-ONOO has excellent potential for further study ONOO-related inflammatory diseases.
细胞内过氧亚硝酸盐阴离子 (ONOO) 水平的变化与炎症的发生和发展密切相关。在炎症部位特异性成像 ONOO 不仅对炎症诊断具有重要意义，而且可以更深入地了解 ONOO 在炎症中的作用。因此，迫切需要构建一些可靠的工具来研究生物体系中 ONOO 与炎症的关系。在这项工作中，我们开发了一种稳健的高选择性荧光开启纳米探针 (Rhb-ONOO)，用于炎症靶向的 ONOO 成像。Rhb-ONOO 通过两亲性 Rhb-ONOO 的自组装获得，该探针由疏水性、ONOO 响应性和深红色发光荧光团 (Rhb) 与亲水性生物聚合物乙二醇壳聚糖 (GC) 的缩合反应构建而成。Rhb-ONOO 对 ONOO 的响应迅速，在 60s 内即可完成，荧光强度比 (I/I) 增强了 19 倍，具有很高的灵敏度，对 ONOO 具有优异的选择性，对其他分析物也具有良好的选择性，并且在 0-1μM 的 ONOO 浓度范围内观察到良好的线性关系，检测限 (LOD) 低至 33nM。令人印象深刻的是，成功地将 Rhb-ONOO 用于活炎症细胞和药物诱导的炎症小鼠中的 ONOO 成像，表明纳米探针 Rhb-ONOO 具有进一步研究与 ONOO 相关的炎症性疾病的巨大潜力。
