Gao Xueke, Zhao Likang, Zhu Xiangzhen, Wang Li, Zhang Kaixin, Li Dongyang, Ji Jichao, Niu Lin, Luo Junyu, Cui Jinjie
Zhengzhou Reseach Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 455001, Zhengzhou, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, 831100, Changji, China.
Zhengzhou Reseach Base, State Key Laboratory of Cotton Biology, Zhengzhou University, 455001, Zhengzhou, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China.
Ecotoxicol Environ Saf. 2023 Apr 15;255:114785. doi: 10.1016/j.ecoenv.2023.114785. Epub 2023 Mar 17.
Assessing the potential effects of insecticides on beneficial biological control agents is key to facilitating the success of integrated pest management (IPM) approaches. Flupyradifurone (FPF) is a novel neonicotinoid insecticide that is replacing traditional neonicotinoids over a large geographical range to control pests. Binodoxys communis, is the dominant parasitic natural enemy of aphids. To date, no reports have addressed sublethal effects of FPF on B. communis. In this study, the lethal and sublethal effects of FPF on B. communis were investigated by indirect exposure to larvae and direct exposure to adults. Results showed that the sublethal LC and LC of FPF had negative effects on the biological parameters of B. communis, including significantly reducing survival rate, adult longevity, parasitism rate, and emergence rate, and significantly prolonging the developmental stages from egg to cocoons. In addition, we observed a transgenerational effect of FPF on the next generation (F1). RNA-Seq transcriptomic analysis identified a total of 1429 differentially expressed genes (DEGs) that were significantly changed between FPF-treated and control groups. These DEGs are mainly enriched in metabolic pathways such as peroxisomes, glutamate metabolism, carbon metabolism, fatty acid metabolism, and amino acid metabolism. This report is the first comprehensive evaluation of how FPF effects B. communis, which adds to the methods of assessing pesticide exposure in parasitic natural enemies. We speculate that the significant changes in pathways, especially those related to lipid synthesis, may be the reason for weakened parasitoid biocontrol ability. The present study provides new evidence for the toxic effects and environmental residue risk of FPF.
评估杀虫剂对有益生物防治剂的潜在影响是促进综合虫害管理（IPM）方法成功的关键。氟吡呋喃酮（FPF）是一种新型烟碱类杀虫剂，正在大范围取代传统烟碱类杀虫剂以控制害虫。豆柄瘤蚜茧蜂是蚜虫的主要寄生性天敌。迄今为止，尚无关于FPF对豆柄瘤蚜茧蜂亚致死效应的报道。在本研究中，通过间接暴露于幼虫和直接暴露于成虫来研究FPF对豆柄瘤蚜茧蜂的致死和亚致死效应。结果表明，FPF的亚致死LC和LC对豆柄瘤蚜茧蜂的生物学参数具有负面影响，包括显著降低存活率、成虫寿命、寄生率和羽化率，并显著延长从卵到茧的发育阶段。此外，我们观察到FPF对下一代（F1）有跨代效应。RNA-Seq转录组分析共鉴定出1429个差异表达基因（DEG），这些基因在FPF处理组和对照组之间有显著变化。这些DEG主要富集在过氧化物酶体、谷氨酸代谢、碳代谢、脂肪酸代谢和氨基酸代谢等代谢途径中。本报告是对FPF如何影响豆柄瘤蚜茧蜂的首次全面评估，增加了评估寄生性天敌农药暴露的方法。我们推测，途径的显著变化，尤其是与脂质合成相关的途径，可能是寄生蜂生物防治能力减弱的原因。本研究为FPF的毒性效应和环境残留风险提供了新的证据。
