Du Lingen, Zhao Likang, Elumalai Punniyakotti, Zhu Xiangzhen, Wang Li, Zhang Kaixin, Li Dongyang, Ji Jichao, Luo Junyu, Cui Jinjie, Gao Xueke
National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Environ Sci Pollut Res Int. 2024 Jan 31. doi: 10.1007/s11356-024-32211-6.
The effective systemic insecticide fipronil is widely used on a variety of crops and in public spaces to control insect pests. Binodoxys communis (Gahan) (Hymenoptera: Braconidae) is the dominant natural enemy of Aphis gossypii Glover (Homoptera: Aphididae), an important cotton pest, and has good efficiency in inhibiting aphid populations. The direct effects of environmental residues of sublethal fipronil doses on adult B. communis have not previously been reported. This study therefore aimed to evaluate the side effects and transcriptomic impacts of sublethal fipronil doses on B. communis. The results showed that exposure to the LC dose of fipronil significantly reduced the survival rate and parasitism rate of the F generation, but did not affect these indicators in the F generation. The LC dose did not affect the survival or parasitic rates of the F generation, but did significantly reduce the survival rate of F generation parasitoids. These results indicated that sublethal doses of fipronil affected B. communis population growth. Transcriptome analysis showed that differentially expressed genes (DEGs) in B. communis at 1 h after treatment were primarily enriched in pathways associated with fatty acid elongation, biosynthesis of fatty acids, and fatty acid metabolism. DEGs at 3 days after treatment were mainly enriched in ribosomal functions, glycolysis/gluconeogenesis, and tyrosine metabolism. Six DEGs (PY, ELOVL, VLCOAR, MRJP1, ELOVL AAEL008004-like, and RPL13) were selected for validation with real-time fluorescent quantitative PCR. This is the first report of sublethal, trans-generational, and transcriptomic side effects of fipronil on the dominant parasitoid of A. gossypii. The results of this study show that adaptation of parasitoids to high concentrations of pesticides may be at the expense of their offspring. These findings broaden our overall understanding of the intergenerational adjustments used by insects to respond to pesticide stress and call for risk assessments of the long-term impacts and intergenerational effects of other pesticides.
高效的系统性杀虫剂氟虫腈被广泛用于多种农作物和公共场所来防治害虫。棉蚜茧蜂（Gahan）（膜翅目：茧蜂科）是棉蚜（半翅目：蚜科）的主要天敌，棉蚜是一种重要的棉花害虫，棉蚜茧蜂在抑制蚜虫种群数量方面具有良好效果。之前尚未报道过亚致死剂量的氟虫腈环境残留对成年棉蚜茧蜂的直接影响。因此，本研究旨在评估亚致死剂量的氟虫腈对棉蚜茧蜂的副作用和转录组影响。结果表明，暴露于氟虫腈的LC剂量显著降低了F1代的存活率和寄生率，但对F2代的这些指标没有影响。LC50剂量对F2代的存活率或寄生率没有影响，但显著降低了F3代寄生蜂的存活率。这些结果表明，亚致死剂量的氟虫腈影响了棉蚜茧蜂的种群增长。转录组分析表明，处理后1小时棉蚜茧蜂中差异表达基因（DEGs）主要富集在与脂肪酸延长、脂肪酸生物合成和脂肪酸代谢相关的途径中。处理后3天的DEGs主要富集在核糖体功能、糖酵解/糖异生和酪氨酸代谢中。选择了6个DEGs（PY、ELOVL、VLCOAR、MRJP1、ELOVL AAEL008004-like和RPL13）用于实时荧光定量PCR验证。这是关于氟虫腈对棉蚜主要寄生蜂的亚致死、跨代和转录组副作用的首次报道。本研究结果表明，寄生蜂对高浓度农药的适应可能以其后代为代价。这些发现拓宽了我们对昆虫用于应对农药胁迫的代际调节的总体理解，并呼吁对其他农药的长期影响和代际效应进行风险评估。
