用于包装海鲜腐败检测的双信号荧光试纸：挥发性盐基氮的可视化监测
Dual-signal fluorescent test strips for spoilage sensing of packaged seafood: Visual monitoring of volatile basic nitrogens.
作者信息
Zhang Wenyang, Ma Ji, Sun Da-Wen
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China.
出版信息
Food Chem. 2023 Aug 1;416:135725. doi: 10.1016/j.foodchem.2023.135725. Epub 2023 Feb 21.
With the food safety issues abounding, exploring reliable and efficient methods for evaluating food safety is crucial. Herein, a ratiometric test strip was proposed by using green-yellow fluorescent d-penicillamine capped silver nanocluster (DPA-AgNCs) as indicator and red-emitting bimetallic gold/silver nanoclusters (AuAgNCs) as an internal reference, providing a real-time and visual monitoring system for food freshness. Results showed that the as-prepared DPA-AgNCs displayed an excellent response and good sensitivity for volatile basic nitrogens (VBNs), with a limit of detection (LOD) of 0.51 μM and 0.08 ppm for spermidine and ammonia hydroxide, respectively. Subsequently, a ratiometric test strip was developed to visually monitor ammonia vapour, displaying an obvious fluorescence colour variation from mustard to deep-red. Moreover, the presented ratiometric test strip was successfully applied for non-contact and visual evaluating and monitoring VBNs in the shrimp sample, showing high potential for in-situ monitoring.
随着食品安全问题层出不穷，探索可靠且高效的食品安全评估方法至关重要。在此，提出了一种比率型测试条，以绿色-黄色荧光的d-青霉胺包覆银纳米簇（DPA-AgNCs）为指示剂，以发红光的双金属金/银纳米簇（AuAgNCs）作为内参，为食品新鲜度提供了一种实时可视化监测系统。结果表明，所制备的DPA-AgNCs对挥发性盐基氮（VBNs）表现出优异的响应和良好的灵敏度，对亚精胺和氢氧化铵的检测限（LOD）分别为0.51 μM和0.08 ppm。随后，开发了一种比率型测试条用于可视化监测氨气，显示出从芥末黄到深红色的明显荧光颜色变化。此外，所提出的比率型测试条成功应用于虾样品中VBNs的非接触式可视化评估和监测，展现出原位监测的巨大潜力。
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