用于神经节苷脂高效薄层色谱和高效液相色谱的新型溶剂系统。
New solvent system for high-performance thin-layer chromatography and high-performance liquid chromatography of gangliosides.
作者信息
Ando S, Waki H, Kon K
机构信息
Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
出版信息
J Chromatogr. 1987 Sep 18;405:125-34. doi: 10.1016/s0021-9673(01)81754-4.
New solvent systems consisting of acetonitrile, isopropanol and aqueous 50 mM potassium chloride or 2.5 M ammonium hydroxide were developed for the separation of gangliosides by high-performance thin-layer chromatography. These solvent systems seem to be superior for the resolution of polysialogangliosides such as tetra-, penta- and hexasialo species, as compared to chloroform-methanol-aqueous salt systems. The order of mobility of gangliosides in the ammoniacal solvent system is GD3 greater than GD1a greater than GM1 greater than GT1b greater than GD1b as compared with GM1 greater than GD3, GD1a greater than GD1b greater than GT1b in the neutral septem. A combination of these two solvent systems provides excellent two-dimensional separations of complex ganglioside mixtures. The neutral solvent system, acetonitrile-isopropanol-aqueous 50 mM potassium chloride, can be used for the separation of underivatized gangliosides by high-performance liquid chromatography on an Aquasil SS silica gel column. Ganglioside elution can be monitored at 208 nm because of the good UV-transparency of the effluent.
开发了由乙腈、异丙醇和50 mM氯化钾水溶液或2.5 M氢氧化铵组成的新型溶剂体系，用于通过高效薄层色谱法分离神经节苷脂。与氯仿 - 甲醇 - 盐水体系相比，这些溶剂体系在分离多唾液酸神经节苷脂（如四唾液酸、五唾液酸和六唾液酸种类）方面似乎更具优势。在氨性溶剂体系中，神经节苷脂的迁移顺序为GD3大于GD1a大于GM1大于GT1b大于GD1b，而在中性体系中为GM1大于GD3，GD1a大于GD1b大于GT1b。这两种溶剂体系的组合可对复杂的神经节苷脂混合物进行出色的二维分离。中性溶剂体系，即乙腈 - 异丙醇 - 50 mM氯化钾水溶液，可用于在Aquasil SS硅胶柱上通过高效液相色谱法分离未衍生化的神经节苷脂。由于流出物具有良好的紫外透明度，因此可在208 nm处监测神经节苷脂的洗脱。
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