Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, 510515 Guangzhou, China; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, 510515 Guangzhou, China.
Forensic Sci Int. 2023 May;346:111637. doi: 10.1016/j.forsciint.2023.111637. Epub 2023 Mar 13.
The insertion/deletion (InDel) polymorphism has promising applications in forensic DNA analysis. However, the insufficient forensic efficiencies of the present InDel-based systems restrict their applications in parentage testing, due to the lower genetic polymorphism of the biallelic InDel locus and the limited number of InDel loci in a multiplex amplification system. Here, we introduced an in-house developed system which contained 41 polymorphic Multi-InDel markers (equivalent to 82 InDels in total), to serve as an efficient and reliable tool for different forensic applications in the Manchu and Mongolian groups. We demonstrated that the new system exhibited potential efficiencies for personal identification, parentage testing, two-person DNA mixture interpretation and ancestry inference of intercontinental populations. Meanwhile, we explored the genetic backgrounds of the Manchu and Mongolian groups by conducting a series of population genetic analyses. We showed that the Manchu and Mongolian groups shared closer genetic relationships with East Asian populations, especially Han Chinese populations in northern China. Moreover, more similar genetic compositions were detected between the Manchu group and the northern Han populations in this study, suggesting that the Manchu group had higher genetic affinities with northern Han populations than the Mongolian group. Overall. this study provided the necessary evidence that these Multi-InDel genetic markers could play an important role in forensic applications.
插入/缺失（InDel）多态性在法医 DNA 分析中具有广阔的应用前景。然而，由于双等位基因 InDel 位点的遗传多态性较低，以及多重扩增系统中 InDel 位点的数量有限，目前基于 InDel 的系统在亲子鉴定中的应用效率不足，限制了其应用。本研究引入了一种自主研发的系统，其中包含 41 个多态性的多等位基因 InDel 标记物（相当于总共 82 个 InDels），作为满族和蒙古族群体中不同法医应用的有效和可靠工具。我们证明了该新系统在个人识别、亲子鉴定、两人 DNA 混合解释和洲际人群的祖籍推断方面具有潜在的效率。同时，我们通过一系列群体遗传分析探讨了满族和蒙古族群体的遗传背景。结果表明，满族和蒙古族群体与东亚人群，特别是中国北方的汉族人群，具有更密切的遗传关系。此外，在本研究中，满族群体与北方汉族群体之间检测到更相似的遗传组成，表明满族群体与北方汉族群体的遗传亲和力高于蒙古族群体。总之，本研究提供了必要的证据，证明这些多等位基因 InDel 遗传标记物可以在法医应用中发挥重要作用。
