Jin Yaqi, Jin Zihui, Sun Di, Peng Yilin, Zhao Qiyang, He Yue, Li Jing, Zhang Yaohai, Cui Yongliang
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China; National Citrus Engineering Research Center, Chongqing 400712, China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing 400712, China.
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China; National Citrus Engineering Research Center, Chongqing 400712, China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing 400712, China.
Food Chem. 2023 Aug 15;417:135936. doi: 10.1016/j.foodchem.2023.135936. Epub 2023 Mar 13.
Spirodiclofen, a spirocyclic tetronic acid derivative, has excellent acaricidal effect and is used worldwide to control the majority of important mite species. For monitoring its residue in food and environmental samples, two haptens containing different spacer arms were synthesized, a monoclonal antibody (mAb 5A4) against spirodiclofen was prepared, and a heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established. The 50% inhibition concentration (IC) of ic-ELISA was 25.46 ng/mL, and the working range was 5.59-133.85 ng/mL. The ic-ELISA showed no cross-reactivity with structural analogs of spirodiclofen and other commonly-used acaricides. The average recoveries from Shiranui citrus samples and Yangtze River water were 85.62%-97.74% and 85.95%-99.30%, respectively. In the analysis of 12 citrus samples, the results of the ic-ELISA were quite similar to those of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Hence, the new immunosorbent assay provides a substitute method for the qualitative and quantitative of spirodiclofen in food and environmental samples.
螺螨酯是一种螺环四酮酸衍生物，具有优异的杀螨效果，在全球范围内用于防治大多数重要的螨类物种。为监测其在食品和环境样品中的残留，合成了两种含有不同间隔臂的半抗原，制备了抗螺螨酯的单克隆抗体（mAb 5A4），并建立了异源间接竞争酶联免疫吸附测定法（ic-ELISA）。ic-ELISA的半数抑制浓度（IC）为25.46 ng/mL，工作范围为5.59 - 133.85 ng/mL。该ic-ELISA与螺螨酯的结构类似物及其他常用杀螨剂无交叉反应。不知火柑橘样品和长江水的平均回收率分别为85.62% - 97.74%和85.95% - 99.30%。在对12个柑橘样品的分析中，ic-ELISA的结果与超高效液相色谱 - 串联质谱法（UPLC-MS/MS）的结果非常相似。因此，这种新的免疫吸附测定法为食品和环境样品中螺螨酯的定性和定量分析提供了一种替代方法。
