Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, China; National Citrus Engineering Research Center, Chongqing 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing 400712, China.
Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, China.
Food Chem. 2024 Sep 30;453:139697. doi: 10.1016/j.foodchem.2024.139697. Epub 2024 May 17.
Spiropidion developed by Syngenta shows high insecticidal and acaricidal activity against a wide range of sucking pests. In this study, according to the structure of spiropidion, two haptens were synthesized by introducing carboxyl groups from the ester group. After cell fusion, a monoclonal antibody (mAb 8B5) of spiropidion was obtained. The IC of the established heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was 7.36 ng/mL, and its working range was 1.75-34.92 ng/mL. The average recoveries were 76.05-124.78% in the Yangtze River and citrus samples. Moreover, the ic-ELISA results of 15 citrus samples agreed well with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Overall, the established ic-ELISA could be applied for the spiropidion residue monitor in food and agricultural samples.
先正达公司开发的螺虫乙酯对广泛的刺吸式害虫具有高效的杀虫和杀螨活性。本研究根据螺虫乙酯的结构，通过酯基引入羧基基团合成了两种半抗原。经过细胞融合，获得了螺虫乙酯的单克隆抗体（mAb8B5）。建立的异源间接竞争酶联免疫吸附测定（ic-ELISA）的 IC 为 7.36ng/mL，其工作范围为 1.75-34.92ng/mL。在长江和柑橘样品中的平均回收率为 76.05-124.78%。此外，15 份柑橘样品的 ic-ELISA 结果与超高效液相色谱-串联质谱（UPLC-MS/MS）吻合良好。总的来说，建立的 ic-ELISA 可用于食品和农业样品中螺虫乙酯残留的监测。
