Antioxidant, antibacterial, and anti-inflammatory Periplaneta americana remnant chitosan/polysaccharide composite film: In vivo wound healing application evaluation.

Periplaneta americana (P. americana), which is widely used for wound healing in China, produces a large amount of solid waste (P. americana remnant) after pharmaceutical production extraction. P. americana remnant chitosan (PAC) has a low molecular weight, low crystallinity, and easily modifiable structural properties. In this study, PAC and P. americana remnant polysaccharide (PAP) were used as raw materials to prepare a composite film (PAPCF). The good biocompatibility of the composite film was verified by cell proliferation assays and protein adsorption assays. The bioactivity of the composite film was assessed by antibacterial and in vivo/vitro antioxidant assays to evaluate its potential as a wound dressing. The wound healing experiment revealed that PAPCF improved wound closure and collagen deposition, decreased reactive oxygen species levels, and attenuated the inflammatory response, enabling rapid wound healing from the inflammatory phase to the proliferative phase in mice. Additionally, PAPCF was administered only once, reducing the chance of infection from multiple deliveries. In summary, this paper presents an easy-to-administer, cost-effective, and effective dressing candidate for wound treatment based on the environmental concept of resource reuse.