State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu, 610065, China.
Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Macromol Biosci. 2020 Mar;20(3):e1900385. doi: 10.1002/mabi.201900385. Epub 2020 Feb 14.
Rapid absorption of wound exudate and prevention of wound infection are prerequisites for wound dressing to accelerate wound healing. In this study, a novel kind of promising wound dressing is developed by incorporating polyhexamethylene guanidine (PHMG)-modified graphene oxide (mGO) into the poly(vinyl alcohol)/chitosan (PVA/CS) matrix, conferring the dressing the required mechanical properties, higher water vapor transmission rate (WVTR), less swelling time, improved antibacterial activity, and more cell proliferation compared to the PVA/CS film crosslinked by genipin. In vivo experiments indicate that the PVA/CS/mGO composite film can accelerate wound healing via enhancement of the re-epithelialization. PVA/CS/mGO composite film with 0.5 wt% mGO sheets displays the best wound healing properties, as manifested by the 50% higher antibacterial rate compared to GO and the wound healing rate of the mouse using this dressing is about 41% faster than the control group and 31% faster than the pure PVA/CS dressing. The underlying mechanism of the accelerated wound healing properties may be a result of the improved antibacterial ability to eradicate pathogenic bacteria on the wound area and maintain an appropriate moist aseptic wound healing environment to accelerate re-epithelialization. These findings suggest that this novel composite PVA/CS/mGO film may have promising applications in wound dressing.
迅速吸收伤口渗出物和预防伤口感染是伤口敷料加速伤口愈合的前提条件。在这项研究中，通过将聚六亚甲基胍（PHMG）改性氧化石墨烯（mGO）掺入聚乙烯醇/壳聚糖（PVA/CS）基质中，开发出了一种新型有前途的伤口敷料，与京尼平交联的 PVA/CS 膜相比，敷料具有所需的机械性能、更高的水蒸气透过率（WVTR）、更短的膨胀时间、更好的抗菌活性和更高的细胞增殖能力。体内实验表明，PVA/CS/mGO 复合膜通过促进再上皮化来加速伤口愈合。含有 0.5wt%mGO 片的 PVA/CS/mGO 复合膜显示出最佳的伤口愈合性能，其抗菌率比 GO 高 50%，使用这种敷料的小鼠的伤口愈合速度比对照组快约 41%，比纯 PVA/CS 敷料快 31%。加速伤口愈合性能的潜在机制可能是由于抗菌能力的提高，能够消灭伤口区域的致病细菌，并保持适当的湿润无菌伤口愈合环境，从而加速再上皮化。这些发现表明，这种新型复合 PVA/CS/mGO 膜可能在伤口敷料中有很好的应用前景。
