Khan Naqeeb Ullah, Sajid Muhammad, Bibi Saira, Rehman Wajid, Alanazi Mohammed M, Abdellatif Magda H
Department of Biochemistry, Hazara University, Mansehra 21300, Khyber Pakhtunkhwa, Pakistan.
Department of Chemistry, Hazara University, Mansehra 21300, Khyber Pakhtunkhwa, Pakistan.
ACS Omega. 2023 Feb 16;8(10):9454-9463. doi: 10.1021/acsomega.2c08124. eCollection 2023 Mar 14.
Considering foremost global issues instigated by parasitic nematodes, () and () nematicidal potential at the gene level has been explored herein. Methanol, ethyl acetate, chloroform, -hexane, and distilled water were used for extract preparation. () was used as the model organism. Nematicidal and anti-egg hatching assays, fluorescence microscopy, and quantitative real-time PCR were done. chloroform (LD = 1.21 mg/mL) and methanol (LD = 2.47 mg/mL) extracts exhibited excellent nematicidal potential. Both plants showed potent anti-egg hatching activity (1 mg/mL). methanol and ethyl acetate extracts showed high apoptotic effect in muscles, gonads, and uterus (eggs). Stress genes, that is, -4, -16.2, and -1 were highly expressed in affected (treated with and leaf extracts) when compared with normal . Phytochemicals and bioactive compounds present in plants may be the major cause of their excellent nematicidal potential, which further confirmed that both plants could be an alternative candidate(s) for novel broad-scale anthelmintic drug(s).
考虑到寄生线虫引发的首要全球问题，本文在基因水平上探索了（）和（）的杀线虫潜力。使用甲醇、乙酸乙酯、氯仿、正己烷和蒸馏水制备提取物。（）用作模式生物。进行了杀线虫和抗卵孵化试验、荧光显微镜检查和定量实时聚合酶链反应。氯仿（半数致死剂量=1.21毫克/毫升）和甲醇（半数致死剂量=2.47毫克/毫升）提取物表现出优异的杀线虫潜力。两种植物均表现出强大的抗卵孵化活性（1毫克/毫升）。甲醇和乙酸乙酯提取物在肌肉、性腺和子宫（卵）中显示出高凋亡效应。与正常（）相比，应激基因，即-4、-16.2和-1在受影响的（用（）和（）叶提取物处理）中高度表达。植物中存在的植物化学物质和生物活性化合物可能是其优异杀线虫潜力的主要原因，这进一步证实这两种植物可能是新型广谱驱虫药物的替代候选物。
