Nematicidal Characterization of and Leaf Extracts Using as a Model Organism.

Considering foremost global issues instigated by parasitic nematodes, () and () nematicidal potential at the gene level has been explored herein. Methanol, ethyl acetate, chloroform, -hexane, and distilled water were used for extract preparation. () was used as the model organism. Nematicidal and anti-egg hatching assays, fluorescence microscopy, and quantitative real-time PCR were done. chloroform (LD = 1.21 mg/mL) and methanol (LD = 2.47 mg/mL) extracts exhibited excellent nematicidal potential. Both plants showed potent anti-egg hatching activity (1 mg/mL). methanol and ethyl acetate extracts showed high apoptotic effect in muscles, gonads, and uterus (eggs). Stress genes, that is, -4, -16.2, and -1 were highly expressed in affected (treated with and leaf extracts) when compared with normal . Phytochemicals and bioactive compounds present in plants may be the major cause of their excellent nematicidal potential, which further confirmed that both plants could be an alternative candidate(s) for novel broad-scale anthelmintic drug(s).