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在非水溶剂中使用比色硫醇-二硫化物交换进行硫醇定量分析。

Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents.

作者信息

Schmidt Ryan, Logan Matthew G, Patty Sarah, Ferracane Jack L, Pfeifer Carmem S, Kendall Alexander J

机构信息

Biomaterials and Biomechanics, School of Dentistry, Oregon Health & Sciences University, 2730 S Moody Ave., Portland, Oregon 97201, United States.

出版信息

ACS Omega. 2023 Feb 28;8(10):9356-9363. doi: 10.1021/acsomega.2c07792. eCollection 2023 Mar 14.

DOI:10.1021/acsomega.2c07792
PMID:36936287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10018523/
Abstract

A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores' behavior (Ellman's reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.

摘要

本文对两种（硫醇-二硫键交换）硫醇定量发色团（埃尔曼试剂和醛硫醇-4）在非水溶剂中的行为进行了仔细分析。测量了广泛的动力学曲线和响应因子，以显示非水体系存在很大差异。与水性条件相比，我们报告了几种使用不同有机溶剂的稳健的台式和室温方法。还介绍了非水体系中分析方法的验证以及卵清蛋白半胱氨酸含量的定量。这项工作是关于在非水条件下利用硫醇-二硫键交换发色团定量硫醇的一篇论文。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/c20f65d24f0f/ao2c07792_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/67444737920b/ao2c07792_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/0133036ef3d5/ao2c07792_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/16f89473fe74/ao2c07792_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/754dff54e1df/ao2c07792_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/83c3cf76fecf/ao2c07792_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/ae8826a761d7/ao2c07792_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/c20f65d24f0f/ao2c07792_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/67444737920b/ao2c07792_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/0133036ef3d5/ao2c07792_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/16f89473fe74/ao2c07792_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/754dff54e1df/ao2c07792_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/83c3cf76fecf/ao2c07792_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/ae8826a761d7/ao2c07792_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c23/10018523/c20f65d24f0f/ao2c07792_0007.jpg

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