新型硫醇敏感性染料在细胞培养物氧化应激测量中的应用。
New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures.
机构信息
Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Cartuja Campus, 18071, Granada, Spain.
Department of Organic Chemistry, Faculty of Sciences, University of Granada, C. U. Fuentenueva s/n, 18071, Granada, Spain.
出版信息
Sci Rep. 2019 Feb 7;9(1):1659. doi: 10.1038/s41598-018-38132-y.
A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.
一种呫吨衍生物，Granada Green 二硝基苯磺酸盐（GGDNBS），已被合成用于基于生物硫醇浓度变化来检测细胞氧化应激。该染料能够通过硫醇解反应与生物硫醇反应，从而促进荧光强度的变化。为了证明 GGDNBS 用于体内氧化应激测量的有用性，将 661 W 光感受器衍生细胞暴露于光下以诱导 ROS 的产生，并测量 GGDNBS 荧光的变化。在这些细胞中，GGDNBS 荧光与通过酶法测量的生物硫醇水平相关。因此，GGDNBS 允许我们通过单细胞生物成像来监测与 ROS 生成相关的生物硫醇水平的变化。
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