Carvajal Romina I, Silva-Mieres Fabiola, Ilabaca Alejandra, Rocha Jorge, Arellano-Arriagada Luciano, Zuniga Arbalti Felipe A, García-Cancino Apolinaria
Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Víctor Lamas 1290, Concepción 4030000, Chile.
Facultad de Ciencias de la Naturaleza, Universidad San Sebastián, Sede Concepción, Lientur 1457, Concepción 4030000, Chile.
Saudi J Biol Sci. 2023 Apr;30(4):103612. doi: 10.1016/j.sjbs.2023.103612. Epub 2023 Mar 2.
Considering the economic and environmental role played by bees and their present threats it is necessary to develop food supplements favoring their health. The aim of this work was to isolate and characterize an immunomodulating probiotic capable to improve the health of honeybee colonies. For this purpose, bacterial strains were isolated from bees (N = 180) obtained at three apiaries. A total of 44 strains were isolated and 9 of them were identified as having the capacity to grow under saccharose osmotic stress, at pH 4.0 and possessing a wide susceptibility to antibiotics. Results allowed to select two strains but finally only one of them, strain A14.2 showed a very significant immunomodulating activity. This strain increased the expression of mRNA codifying the antimicrobial peptides 24 h post-administration. We evaluated its growth kinetics under aerobic and microaerobic conditions and its survival in the presence of high concentrations of saccharose. Results demonstrated that A14.2 strain was highly tolerant to oxygen and that it was able to adapt to saccharose enriched environments (50% and 100% w/v). Finally, . A14.2 strain was administered monthly during summer and early fall to 4 honeybee colonies (2 controls and 2 treatments). The results showed a gradual sustained decrease of infestation ( < 0.05) by the pathogenic spp. but no reduction in the infestation by the mite . These results suggest that the administration of this potential probiotic, may increase the resistance of honeybee colonies to infectious diseases caused by spp.
考虑到蜜蜂所发挥的经济和环境作用及其当前面临的威胁，有必要开发有利于其健康的食品补充剂。这项工作的目的是分离和鉴定一种能够改善蜂群健康的免疫调节益生菌。为此，从三个养蜂场采集的蜜蜂（N = 180）中分离出细菌菌株。共分离出44株菌株，其中9株被鉴定为能够在蔗糖渗透胁迫下、pH 4.0条件下生长，并且对多种抗生素具有广泛的敏感性。结果筛选出两株菌株，但最终只有其中一株，即A14.2菌株表现出非常显著的免疫调节活性。该菌株在给药后24小时增加了编码抗菌肽的mRNA的表达。我们评估了其在有氧和微需氧条件下的生长动力学以及在高浓度蔗糖存在下的存活情况。结果表明，A14.2菌株对氧气具有高度耐受性，并且能够适应富含蔗糖的环境（50%和100% w/v）。最后，在夏季和初秋每月对4个蜂群（2个对照和2个处理组）施用A14.2菌株。结果显示，致病的spp.感染率逐渐持续下降（<0.05），但螨感染率没有降低。这些结果表明，施用这种潜在的益生菌可能会增加蜂群对由spp.引起的传染病的抵抗力。
