College of Veterinary Medicine, Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, Qingdao Agricultural University, Qingdao, China.
Animal Husbandry and Veterinary Station of Rushanzhai Town, Rushan Animal Husbandry Development Center, Weihai, China.
Front Cell Infect Microbiol. 2023 Mar 1;13:1142173. doi: 10.3389/fcimb.2023.1142173. eCollection 2023.
Porcine epidemic diarrhea virus (PEDV), an intestinal pathogenic coronavirus, has caused significant economic losses to the swine industry worldwide. At present, there are several treatment methods, but there is still a lack of clinically effective targeted drugs, new antiviral mechanisms and drugs need to be explored.
In this study, we established a model of erastin versus ferrostatin-1 treatment of Vero cells, and then detected virus proliferation and gene expression by RT-qPCR through PEDV infection experiments.
We demonstrated for the first time that erastin significantly inhibited the replication of PEDV upon entry into cells; Vero treated with erastin significantly regulated the expression of three genes, , and , notably erastin regulated the expression of these three genes negatively correlated with the expression induced by PEDV virus infection.
Since , and are classical Ferroptosis genes, this study speculates that erastin may inhibit the replication of PEDV in Vero cells in part through the regulation of ferroptosis pathway, and erastin may be a potential drug for the treatment of PEDV infection.
猪流行性腹泻病毒（PEDV）是一种肠道致病性冠状病毒，已在全球范围内给养猪业造成了重大经济损失。目前有几种治疗方法，但仍然缺乏临床有效的靶向药物，需要探索新的抗病毒机制和药物。
本研究建立了依拉司琼与 ferrostatin-1 处理 Vero 细胞的模型，然后通过 PEDV 感染实验，通过 RT-qPCR 检测病毒增殖和基因表达。
我们首次证明依拉司琼在细胞进入时能显著抑制 PEDV 的复制；依拉司琼处理的 Vero 细胞显著调节了三个基因 、 和 的表达，值得注意的是，依拉司琼调节这些三个基因的表达与 PEDV 病毒感染诱导的表达呈负相关。
由于 、 和 是经典的 Ferroptosis 基因，本研究推测依拉司琼可能通过调节铁死亡途径部分抑制 PEDV 在 Vero 细胞中的复制，依拉司琼可能是治疗 PEDV 感染的潜在药物。
