J.F. 米尔种子甲醇提取物及其溶剂馏分的体外抗利什曼原虫活性
In vitro Anti-Leishmanial Activities of Methanol Extract of J.F. Mill Seeds and Its Solvent Fractions.
作者信息
Ketema Tasisa, Tadele Markos, Gebrie Zewdie, Makonnen Eyasu, Hailu Asrat, Abay Solomon M
机构信息
Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia.
Animal Health Research Program, Ethiopian Institute of Agricultural Research, Holetta, Ethiopia.
出版信息
J Exp Pharmacol. 2023 Mar 13;15:123-135. doi: 10.2147/JEP.S397352. eCollection 2023.
INTRODUCTION
Leishmaniasis is one of the neglected tropical diseases, threatening lives of about 350 million people globally. seeds are used for the treatment of cutaneous leishmaniasis in the traditional medicine in Ethiopia.
OBJECTIVE
This study aimed to evaluate seeds' anti-leishmanial activity in vitro.
METHODS
The crude (80% methanol) extract of seeds and its fractions were evaluated for their anti-leishmanial activities against promastigotes and intracellular amastigotes of , and for their cytotoxic effects against mammalian cells. The quantitative estimations of total phenolic compounds (TPCs), flavonoids (TFCs) and alkaloids (TACs) were determined, spectrophotometrically. Median inhibitory concentration (IC) and median cytotoxic concentration (CC) of the extract and its solvent fractions were calculated using GraphPad Prism 9.1.0 computer software. Data was presented as mean ± standard error of the mean (SEM).
RESULTS
The crude extract and its hexane, ethyl acetate and butanol fractions showed anti-leishmanial activities, with IC values of 4.14-60.12 µg/mL against promastigotes, and 6.16-40.12 µg/mL against amastigotes of both species. They showed moderate cytotoxicity against Vero cell lines and peritoneal mice macrophages, with CC values of 100-500 µg/mL, but >1600 µg/mL against red blood cells. Selectivity indices ranged from 7.97 to 30.97. The crude extract, and its ethyl acetate and hexane fractions possessed 54.78-127.72 mg of gallic acid equivalent TPC, 18.30-79.21 mg of quercetin equivalent TFC, and 27.62-97.22 mg of atropine equivalent TAC per gram of extracts.
CONCLUSION
The seeds of the plant possessed anti-leishmanial activities against and that might provide a scientific justification for its use in the treatment of leishmaniasis by traditional healers. Future works are recommended to isolate, purify and identify the possible secondary metabolites attributed to the anti-leishmanial activity.
引言
利什曼病是被忽视的热带疾病之一，全球约3.5亿人的生命受到威胁。在埃塞俄比亚的传统医学中，[植物名称]种子被用于治疗皮肤利什曼病。
目的
本研究旨在评估[植物名称]种子的体外抗利什曼原虫活性。
方法
对[植物名称]种子的粗提物（80%甲醇）及其馏分进行评估，检测其对[利什曼原虫种类]前鞭毛体和细胞内无鞭毛体的抗利什曼原虫活性，以及对哺乳动物细胞的细胞毒性作用。采用分光光度法对总酚类化合物（TPC）、黄酮类化合物（TFC）和生物碱（TAC）进行定量测定。使用GraphPad Prism 9.1.0计算机软件计算提取物及其溶剂馏分的半数抑制浓度（IC）和半数细胞毒性浓度（CC）。数据以平均值±平均标准误差（SEM）表示。
结果
粗提物及其己烷、乙酸乙酯和丁醇馏分均显示出抗利什曼原虫活性，对两种[利什曼原虫种类]前鞭毛体的IC值为4.14 - 60.12 μg/mL，对无鞭毛体的IC值为6.16 - 40.12 μg/mL。它们对Vero细胞系和腹腔小鼠巨噬细胞表现出中度细胞毒性，CC值为100 - 500 μg/mL，但对红细胞的CC值>1600 μg/mL。选择性指数范围为7.97至30.97。粗提物及其乙酸乙酯和己烷馏分每克提取物中含有54.78 - 127.72 mg没食子酸当量的TPC、18.30 - 79.21 mg槲皮素当量的TFC和27. ……
结论
该植物种子对[利什曼原虫种类]具有抗利什曼原虫活性，这可能为传统治疗师将其用于治疗利什曼病提供科学依据。建议未来的研究分离、纯化和鉴定可能具有抗利什曼原虫活性的次生代谢产物。 （注：原文中生物碱定量部分“27.62 - 97.22 mg of atropine equivalent TAC”中“atropine equivalent”可能有误，推测可能是其他生物碱当量，这里按原文翻译）
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