Liu Shucan, Gao Xiankui, Shi Min, Sun Meihong, Li Kunlun, Cai Yan, Chen Chengan, Wang Can, Maoz Itay, Guo Xinhong, Kai Guoyin
Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Jinhua Academy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
College of Biology, Hunan University, Changsha, Hunan 410082, China.
Hortic Res. 2023 Jan 11;10(3):uhad004. doi: 10.1093/hr/uhad004. eCollection 2023 Mar.
Jasmonic acid (JA) signaling pathway plays an important role in tanshinone and phenolic acid biosynthesis in . However, the specific regulatory mechanism remains largely unclear. Previous work showed that a JASMONATE ZIM-domain (JAZ) protein, SmJAZ9, acted as a repressor of tanshinone production in . In this study, we revealed that SmJAZ9 reduced both phenolic acid accumulation and related biosynthetic gene expression, confirming that SmJAZ9 also negatively affected phenolic acid biosynthesis. Then, we identified a novel MYB transcription factor, SmMYB76, which interacted with SmJAZ9. SmMYB76 repressed phenolic acid biosynthesis by directly downregulating , , and . Further investigation demonstrated that JA mediated phenolic acids biosynthesis via SmJAZ9-SmMYB76 complex. Taken together, these findings state the molecular mechanism that SmJAZ9-SmMYB76 regulated phenolic acid biosynthesis at the transcriptional and protein levels, which provided new insights into JA signaling pathway regulating plant metabolism.
茉莉酸（JA）信号通路在丹参酮和酚酸生物合成中起着重要作用。然而，具体的调控机制仍不清楚。先前的研究表明，一种茉莉酸ZIM结构域（JAZ）蛋白SmJAZ9，在丹参中作为丹参酮生成的抑制因子。在本研究中，我们发现SmJAZ9降低了酚酸积累及相关生物合成基因的表达，证实SmJAZ9也对酚酸生物合成产生负面影响。然后，我们鉴定了一个新的MYB转录因子SmMYB76，它与SmJAZ9相互作用。SmMYB76通过直接下调、和来抑制酚酸生物合成。进一步研究表明，JA通过SmJAZ9-SmMYB76复合体介导酚酸生物合成。综上所述，这些发现阐述了SmJAZ9-SmMYB76在转录和蛋白质水平上调控酚酸生物合成的分子机制，为JA信号通路调控植物代谢提供了新的见解。
