Chen Junyu, Ying Yuxin, Yao Lingtiao, Xu Zhangting, Yu Zhenming, Kai Guoyin
School of Pharmaceutical Sciences, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.
College of Food and Health, Zhejiang A & F University, Hangzhou 311300, China.
Plants (Basel). 2024 Feb 21;13(5):580. doi: 10.3390/plants13050580.
is a plant commonly used in traditional Chinese medicine. Its material bases for treating diseases are tanshinones and phenolic acids, including salvianolic acids. Histone deacetylase proteins (HDACs) are a class of specific functional enzymes that interact with acetylation groups on the N-terminal lysine of histone proteins further regulate gene transcription through structural changes at the chromatin level. HDACs involved in the growth and development of various plants, and induced by plant hormones to regulate the internal environment of plants to resist stress, at the same time affect the accumulation of some secondary metabolites. However, the role of SmHDACs on the accumulation of salvianolic acid in remains unclear. In this study, 16 SmHDACs genes were identified from the high-quality genome, their physicochemical properties were predicted. In phylogenetic trees co-constructed with HDACs proteins from other plants, SmHDACs was divided into three subfamilies, each with similar motif and conserved domain composition. The distribution of the three subfamilies is similar to that of dicotyledonous plants. Chromosome localization analysis showed that SmHDACs genes were randomly located. Cis-acting element analysis predicted that SmHDACs gene expression may be related to and induced by various phytohormones, such as MeJA and ABA. By combining the expression pattern and co-expression network induced by phytohormones, we speculate that SmHDACs may further influence the synthesis of salvianolic acid, and identified SmHDA5, a potential functional gene, then speculate its downstream target based on the co-expression network. In summary, we analyzed the SmHDACs gene family of and screened out the potential functional gene . From the perspective of epigenetics, we proposed the molecular mechanism of plant hormone promoting salvianolic acid synthesis, which filled the gap in the subdivision of histone deacetylase in research, provided a theoretical basis for the culture and transformation of germplasm resources.
是一种常用于传统中药的植物。其治疗疾病的物质基础是丹参酮和酚酸，包括丹酚酸。组蛋白去乙酰化酶蛋白（HDACs）是一类特定的功能酶，它们与组蛋白N端赖氨酸上的乙酰化基团相互作用，通过染色质水平的结构变化进一步调节基因转录。HDACs参与各种植物的生长发育，并受植物激素诱导调节植物内部环境以抵抗胁迫，同时影响一些次生代谢产物的积累。然而，SmHDACs在丹参中对丹酚酸积累的作用尚不清楚。在本研究中，从高质量的丹参基因组中鉴定出16个SmHDACs基因，并预测了它们的理化性质。在与其他植物的HDACs蛋白共同构建的系统发育树中，SmHDACs被分为三个亚家族，每个亚家族具有相似的基序和保守结构域组成。这三个亚家族的分布与双子叶植物相似。染色体定位分析表明，SmHDACs基因随机定位。顺式作用元件分析预测，SmHDACs基因表达可能与多种植物激素如茉莉酸甲酯（MeJA）和脱落酸（ABA）有关并受其诱导。通过结合植物激素诱导的表达模式和共表达网络，我们推测SmHDACs可能进一步影响丹酚酸的合成，并鉴定出一个潜在的功能基因SmHDA5，然后根据共表达网络推测其下游靶点。综上所述，我们分析了丹参的SmHDACs基因家族并筛选出潜在的功能基因。从表观遗传学的角度，我们提出了植物激素促进丹酚酸合成的分子机制，填补了丹参研究中组蛋白去乙酰化酶细分领域的空白，为丹参种质资源的培育和转化提供了理论依据。
