Lough J, Rosenthall L, Arzoumanian A, Goresky C A
Department of Pathology, Montreal General Hospital, Quebec, Canada.
J Hepatol. 1987 Oct;5(2):190-8. doi: 10.1016/s0168-8278(87)80572-x.
Rats were made cirrhotic by carbon tetrachloride inhalation associated with phenobarbital in the drinking water over 10 weeks. After a 1-week recovery period a 99mTc sulphur colloid radioisotope scan of the liver was carried out on each animal following bolus injection into the iliac vein. Kupffer cells were then histologically identified by one of three methods: colloidal carbon uptake, iron staining after ferritin-dextran, or endogenous peroxidase staining. The degree of liver injury was classified into four pathological groups and these were correlated with the phagocytic capacity of each liver as recorded on the isotope scan. All three histological markers demonstrated that in normal liver, Kupffer cells are more plentiful in periportal areas. In cirrhotic liver, there were very few Kupffer cells in nodular regenerative areas, where continuous capillaries are found, but Kupffer cells were present in the remaining more normal trabecular-sinusoidal areas. Morphometric counting of carbon- and ferritin-labelled Kupffer cells demonstrated a significant decrease in cirrhotic livers. A close correlation was also found between increasing degree of liver injury and diminished hepatic phagocytic capacity, as demonstrated by the radioisotope scan. The study demonstrates that where regenerative liver is capillarized, with replacement of fenestrated sinusoids, Kupffer cells are absent.
通过吸入四氯化碳并在饮水中添加苯巴比妥，持续10周使大鼠形成肝硬化。在1周的恢复期后，对每只动物经髂静脉推注后进行肝脏的99mTc硫胶体放射性同位素扫描。然后通过三种方法之一进行组织学鉴定库普弗细胞：胶体碳摄取、铁蛋白-葡聚糖后铁染色或内源性过氧化物酶染色。将肝损伤程度分为四个病理组，并将这些与同位素扫描记录的每个肝脏的吞噬能力相关联。所有三种组织学标记物均表明，在正常肝脏中，库普弗细胞在门静脉周围区域更为丰富。在肝硬化肝脏中，在发现连续毛细血管的结节状再生区域中库普弗细胞很少，但在其余更正常的小梁-窦状区域中存在库普弗细胞。对碳和铁蛋白标记的库普弗细胞进行形态计量计数表明，肝硬化肝脏中其数量显著减少。放射性同位素扫描也表明，肝损伤程度增加与肝脏吞噬能力降低之间存在密切相关性。该研究表明，在再生肝脏发生毛细血管化、有孔窦状隙被取代的情况下，不存在库普弗细胞。
